DreINT0025724 @ danRer10
Intron Retention
Gene
ENSDARG00000001431 | actn3b
Description
actinin alpha 3b [Source:ZFIN;Acc:ZDB-GENE-030131-2040]
Coordinates
chr7:6888313-6889902:+
Coord C1 exon
chr7:6888313-6888459
Coord A exon
chr7:6888460-6889836
Coord C2 exon
chr7:6889837-6889902
Length
1377 bp
Sequences
Splice sites
5' ss Seq
AGGGTCAGT
5' ss Score
5.85
3' ss Seq
CTATTGTCATTCACTTGCAGAAG
3' ss Score
8.9
Exon sequences
Seq C1 exon
CACATCCGTGTGGGCTGGGAGCAGCTGCTCACCACCATCGCTCGCACCATCAACGAGGTGGAGAACCAGATCCTGACCCGCGACGCAAAGGGCATCAGCCAGGAGCAGCTCAACGAGTTCAGAGCCTCCTTCAACCACTTCGACAGG
Seq A exon
GTCAGTGAGACGACACACATAGCCAGATTCAGAGCTAAGGCTGCTTGATACTGGAAAAATCTGATTTTATTTTACTTTTATATACATATATATATATATATATATACACACACACACACACACACACACACAATTTCCCCAGATGACTTGAATATCTCTATTTGCATGATTGTGTAGAGGAGTGAATCACGCATGAAATGTAACAAACAAAATTACAAGCATAGATAAATGAAATAGAGCAAAAAAAAATCGAAATAAACAGTGCTTTGTGGTTTTCAGAGTCTAGTCAAATCAAATTCAGGTAGAGAAATACAAAAATTAAATATGAAATAACACTTTAAAGACTTTAAACACTTTCTCATCCACGTTCCTGGACGACCACCAGCTCTGCATGTTTTGGATGTCTCCTTTGTCTGTCACACCCATTACAGGTCTGTCAGTCTCTGCTAATGAGCTGATGATCTGAATCAGGTGTGTTTGGTCACGGAGACATGAAAAATGTGCAGAGCTGGTGGTCCTCCAGGAATGTGGTGGAGAAACACTGCTTTAAACTACTTTTTAAATCAGAGATGCCCAAAGTAGGGCCCACGGGCCAAAGTTAGCCCATTGTAACCACTGATTTGGCCTGCCATCCCATCTGAGAGCAGAGTGAGAATGAAGAAGATGGTTAGAGGCAAATGCCTTCAACACAGAGATCGCCATTTCTAATTTGACAAAACCATTTTTGTATTTTATTGCTGAGCTACAAAAAAGCAAACTCAAGTTAAATGTTTTAATTAAATGTAGATCAATCTGGCACCTTTGAAATGCAGATTAGTAATTAGATTAATTAGATTATAATTAGTTTGTTTTGTTTTTACTGTTATAAGCTCATCATAAAATGTAAAATGTACTGTATTTGTTAATATAAAGCAATGTTTTCTACTCATTTTTAAACATTATTAAATTAATTAGGAAGTTAACCATGTGTTCCTTCAGCCCACAAGCCTCAATCAAGTTTGGTTTTTGTGCCCAACTTTTTCATAGAAATAGCTTTAAATAGTTTAATAAAATACATTTCTATTACAATTACAAATGGTAAAATTTCTTGTGCTTAAATTCTTTACACTCTCCTGGAGCTTAAAACAGGCAAGTGATAAATATTGACTCTGATATGGTTAAACTCTGCAGTAACTCCACAAATCCTATGTATTATAACTGTGGCTATATAATCCCAATATAATATATATAACCCTAAACTATACAATATATATAATAATCACAGACGCTGCGATGTGACTACTGCGGATGTGCCCAATGTTGTGCAGCCATACTGAGAGCTGAGGATGCATGTGTGTGTGTGTGTTATGTGGATTGACTATTGTCATTCACTTGCAG
Seq C2 exon
AAGAGAAACGGCATGATGGACCCCGACGACTTCCGCGCCTGTCTGATCTCTATGGGTTACGATCTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000001431:ENSDART00000001649:18
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (disopred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0043516=Spectrin=PD(20.2=42.9),PF088696=XisI=PD(31.8=42.9),PF134991=EF-hand_7=PU(33.3=53.1)
A:
NA
C2:
PF134991=EF-hand_7=FE(26.9=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATCCGTGTGGGCTGGGAG
R:
TCGTAACCCATAGAGATCAGACAGG
Band lengths:
206-1583
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]