MmuINT0010455 @ mm9
Intron Retention
Gene
ENSMUSG00000006457 | Actn3
Description
actinin alpha 3 [Source:MGI Symbol;Acc:MGI:99678]
Coordinates
chr19:4862170-4862470:-
Coord C1 exon
chr19:4862324-4862470
Coord A exon
chr19:4862236-4862323
Coord C2 exon
chr19:4862170-4862235
Length
88 bp
Sequences
Splice sites
5' ss Seq
CGGGTCGGT
5' ss Score
3.5
3' ss Seq
TATCCATTTGCCCTTGACAGAAG
3' ss Score
6.61
Exon sequences
Seq C1 exon
CACATTCGTGTGGGCTGGGAGCAGTTGCTCACCTCCATTGCCCGCACCATCAATGAGGTCGAGAACCAGGTACTGACCCGAGATGCCAAGGGCCTGAGCCAGGAGCAGCTCAACGAATTCCGCGCCTCCTTCAACCACTTTGACCGG
Seq A exon
GTCGGTGGGGGCTAACTCCTGTGGGCTGACAGACTTGAGGGCGGGTGGGATTTGGAGACCAGACCTGATATCCATTTGCCCTTGACAG
Seq C2 exon
AAGCGGAATGGGATGATGGAGCCCGACGACTTCCGAGCTTGTCTCATCTCCATGGGTTACGATCTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000006457-Actn3:NM_013456:18
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (disopred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0043516=Spectrin=PD(20.2=42.9),PF134991=EF-hand_7=PU(34.2=55.1)
A:
NA
C2:
PF134991=EF-hand_7=FE(26.6=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CAGTTGCTCACCTCCATTGCC
R:
GAGACAAGCTCGGAAGTCGTC
Band lengths:
171-259
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: