DreINT0034163 @ danRer10
Intron Retention
Gene
ENSDARG00000033361 | asl
Description
argininosuccinate lyase [Source:ZFIN;Acc:ZDB-GENE-040426-1152]
Coordinates
chr21:31234729-31235116:+
Coord C1 exon
chr21:31234729-31234812
Coord A exon
chr21:31234813-31235035
Coord C2 exon
chr21:31235036-31235116
Length
223 bp
Sequences
Splice sites
5' ss Seq
AAGGTGAGA
5' ss Score
8.68
3' ss Seq
TATCTTTCCATTTTTTTAAGGTC
3' ss Score
10.29
Exon sequences
Seq C1 exon
GAGGATAAAGAAGCCATGTTTGATACATATGATACTGTACACGCTGTGCTGCAGGTGGCGACAGGCGTCATCTCAACTCTAAAG
Seq A exon
GTGAGAGACGGGTTACATCACAAGTTAATGCAGTTTTATCATGAACAATGGGAATCAAAAGCAAAATGTTACTTTGGTTAAATAATTAATTAAGTGGAATGAGGTATAAACCTGTTGGATGTGTAGTAATCCATTGTCAGGGTGAAATTTGAGCAGCAGGAGTAGTTTTTCTATTAATGGTAGATTCTGCTCGCAATATGTTATATCTTTCCATTTTTTTAAG
Seq C2 exon
GTCAATCAAGTAAAGATGGAAGAAGCCCTCAGTCCAGATATGCTGGCCACTGATCTCGCCTATCATCTGGTCAGAAAAGGA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000033361:ENSDART00000040190:13
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
PF146981=ASL_C2=PU(20.0=51.9)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GAGGATAAAGAAGCCATGTTTGAT
R:
CCTTTTCTGACCAGATGATAGGCG
Band lengths:
164-387
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]