DreINT0046811 @ danRer10
Intron Retention
Gene
ENSDARG00000034307 | chrne
Description
cholinergic receptor, nicotinic, epsilon [Source:ZFIN;Acc:ZDB-GENE-040808-32]
Coordinates
chr5:37843204-37843516:-
Coord C1 exon
chr5:37843402-37843516
Coord A exon
chr5:37843319-37843401
Coord C2 exon
chr5:37843204-37843318
Length
83 bp
Sequences
Splice sites
5' ss Seq
CAAGTCAGT
5' ss Score
2.74
3' ss Seq
CCTTTGTTTCTGTGTTTCAGGTA
3' ss Score
13.03
Exon sequences
Seq C1 exon
CTGGCGGGCAGAAGTTGACTGTGTCCATTTCTGTTCTTTTGGCTCAGACTGTGTTTCTCATCCTGATTTCTCAGAAAATACCAGAAACGTCACTGTCTGTGCCTCTTATTGGCAA
Seq A exon
GTCAGTACCAGTTTTCTCTCTCGACAGTTTCGATTTTTTATCCTTGAATCATCTGATTTCACTCCTTTGTTTCTGTGTTTCAG
Seq C2 exon
GTATTTAATTTTCGTGATGTCTATGACCACACTCATTGTGACCAACTGCATTATTGTGCTCAACTACTCCCTCCGCAGTCCCAGCACACACAACATGTCCCAGAGCATCAAGCAT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000034307:ENSDART00000136428:8
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0293211=Neur_chan_memb=FE(48.7=100)
A:
NA
C2:
PF0293211=Neur_chan_memb=FE(15.6=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Cow
(bosTau6)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGGCTCAGACTGTGTTTCTCA
R:
GACATGTTGTGTGTGCTGGGA
Band lengths:
175-258
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]