DreINT0052431 @ danRer10
Intron Retention
Gene
ENSDARG00000016074 | cryz
Description
crystallin, zeta (quinone reductase) [Source:ZFIN;Acc:ZDB-GENE-050306-24]
Coordinates
chr2:11334378-11335502:-
Coord C1 exon
chr2:11335387-11335502
Coord A exon
chr2:11334531-11335386
Coord C2 exon
chr2:11334378-11334530
Length
856 bp
Sequences
Splice sites
5' ss Seq
CAGGTTGGT
5' ss Score
8.08
3' ss Seq
AACAATTTTTGCTATTATAGGTT
3' ss Score
7.72
Exon sequences
Seq C1 exon
AGACCACCATGGCCGCATCTAGATTAATGAGGGCTGTCAGAGTGTCCGAGTTTGGAGGCCCTTCAGTTTTAAAACTCTGCTCAGACCTGCCTGTACCAAGTCCTGGTCAGAAACAG
Seq A exon
GTTGGTCTCCTAAAAAATACCTTGAATTTTTGATTTCAGATTGTTTGTCTTGAATTAAGTTCTGTTTTCTTCCATAAAAGCATTTTCTGGCAAATTTAGATCACTAAAATGTATGGTTAACCTAAAACAGAAGTGTGTTATTTACTGATCATCATGTACTTGCAAGCACTTGAGACTGCGTACATTAATTATGTTTATATGAATTAAAGCCAAATCTGTTTGTAGCCCTGAAACAACTTAATTCATATGGATTGCATTCACACAGTTTTTTTTTTTTTTTTTTTTGAGCTAACTTTCATGATTGAATGTAAAATTTTGAGTGAACTAACTAAAAAGTCTACCATTTTGGAAATTTCAGAGATAAAGGATGGTTAATTATTGAGACAAATTTGGTTTTATATTCACACATTCGGGTTTTCTCCTATAATTTCTATAAAATTAAAAAAAAATTGTATAATTTCAAAAAAGTATTATTTGCAAATTCTAAATAGTAATATTATATTGCCAATGACTCTTGAAGAACAACATTGTTAACAGTCAAACAAATAGTCCTAATGTTAGTTTGAAGTCATACTTGCATGTAATTTTAGACCCAATATTCAAAGTTCTACAGTAATCAATACAGGGAGCGCTTCAAAAGTTGTCACTGACATGAATGTGCGAATATAAAACCAGTTTCACCTCAATTGGCTGTGTTTCCATAACTAAAAAGTCAAGTATTGTTTGTTACTGTTTAGAAAATGACCCGATTAATTTATGCTTTTAGAAGTTTATATACTTGACTACAATATTAGCTTATTAGCAATTACATCATTGTTTTTCCAAAGATCTTTGTAAACAATTTTTGCTATTATAG
Seq C2 exon
GTTCTCATACGAGTCCATGCATGTGGTGTAAACCCAGTGGAAACATACATCCGCTCTGGATCTTATGCTCGGAAACCCAGTTTGCCCTATACTCCAGGATCAGATGTCTCCGGTGTGGTTGAAGCTGTTGGCGAAGGTGTTTGCCTCCTACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000016074:ENSDART00000131836:2
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion (1st CDS intron)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.020
Domain overlap (PFAM):
C1:
PF082407=ADH_N=PU(3.0=5.6)
A:
NA
C2:
PF082407=ADH_N=FE(75.8=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CACCATGGCCGCATCTAGATT
R:
GTAGGAGGCAAACACCTTCGC
Band lengths:
263-1119
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]