DreINT0054227 @ danRer10
Intron Retention
Gene
ENSDARG00000042956 | cyp2ad6
Description
cytochrome P450, family 2, subfamily AD, polypeptide 6 [Source:ZFIN;Acc:ZDB-GENE-041001-157]
Coordinates
chr20:25629383-25631201:-
Coord C1 exon
chr20:25631041-25631201
Coord A exon
chr20:25629560-25631040
Coord C2 exon
chr20:25629383-25629559
Length
1481 bp
Sequences
Splice sites
5' ss Seq
CAGGTGGAG
5' ss Score
2.57
3' ss Seq
TTTGTTTGTTTGCTTTCCAGCTG
3' ss Score
9.69
Exon sequences
Seq C1 exon
GCCGCCCCTTTGACCCTTTGGTCAAAATCAATAATGCAGTAGCCAACGTTATCGGCTTCCTGGTGTTCGGCCATCGATATGAGTATAGTGATGTTAATTTCCAGAAGCGTCTACAGATGAGTGCGGAGTCAGTGTTTCTTACAGGATCTGTGTGGAACCAG
Seq A exon
GTGGAGTATATTCAAAATACAGCCTATCTATAAAACATTTTGAAATTGTAATCAATTAAAAGAGCATTTAAAGGTGAAATACATACATTTTGTGCACTGCTAGGGTCTTTTAGTGGATTTACCAAAACTAAAACATATTTTCAAACACCTCACTGTAAACTTAACGCACCATTTCTTTAAAGCATTTTATCTTTTTAATGTGTTATTATATTATTTAGGGTTTCAAAGCTAGTTAGTAATAGCTAACTAGTTTATATTATACTGTAAATAACAATTAGTTGACTTAAAAAAGGAATAAACCTGTTGCTATTAAAATGATAAGTTGATTTTGCTTAAAAAGCGAGTAAACTTGTTGCCTTGCATTTAAGTAAATGAACTGACCATAATTATAAAAAAAATAATTAAACATTGGCGATGTGGTGCCTTAGTGGTTAGCACTGTCGCCTCACAGCAAGAGGGCCGCTGGTTCGAGCCCCGGCTGGGTCAGTTGAGCTTCCTAGAGTTTGCATATTCTCCCCATGTTTGCATGGGTTTCCTCTGGGTGCTCCGGTTTCCCCCACAGTCCAAAGACATATGGTATAGGTGAATTCAATAAGCCAAATTGGCCTTAGTATGTGAATGTGAAAGTGTAAGTATGTTTCCCAGTACTGTGTTGCACCTGGAAGGGCATCCGCTGTATAAAACATATGCTGGATAATTTGGTGGTTCATTCTGCTGTGGCAATTCGTGATAAATAAAGGGACTAAGCAGAAGGAAAATTAATTATTAAAGTTAAACATCCAGTCAACTTATTAGTTCCATGTAAACTTGTTGCTTTAAGGCAGGGGGGTCCAAACTTGGTCCTGGAGGGCCGGTATCCTGCAAAGTTTAGTTCCAACCTCAATCAGACACACCTGGGCTAGCTAATCAAGCTCTTAGGTTAAACATCCTTGCAGCTGTGTTGAGGCAAGTTGGAGCTAAAATCTGCAGGACATCAGCCCTCCAGGACCGAATTTAGAAACCCTTACTTTAAGGCAATGACTTACAATATTTTTTAAAGTAATCATATTTTATAGTGTGTCTGTCAGAAAGGAACTCATCAAATGTCAGTATTGATGCTTGTTTCACCTATTGTCTGTCTTTGCAAATTTTGGCAAATCTTCAATTGTAGTCTTTCACTTCGTTATACGTGAATCTGAATGTATTTTGCCTCAGTCATGATCTCACACTTGCTAAAAACCATCTGGCTGAACCTGCTTCAGTAGCCACAGCCCAAGCTCCATGCTATCGGTTGATCTAGAAAGCATTTGGAAGTTTTTAGGGAACCTCTCTTAGTGTACATGCTCTTATAGATGGCATCCTTATCATATTATTGAACATTAATTAAATTTTTACGCATAATTCCCATATATATCTTACAAGATAAGTAGTAAAAAAACGTCAATAGTTGAAACTCAGTAACACTATTTTTAACAAAGCTGTTTTGTTTGTTTGCTTTCCAG
Seq C2 exon
CTGTATGATGCCTTCCCAGGTATAATGAAGTGGGTGCCTGGACCTCATCAGACAATCATTTCAAACTATCAAAAGTTAGCAAACTTTCTGGAAGAGAAGATTGAGCAACATAAACCAGACTGGGACATCAGCAATCCGAGAGATTATATTGATGCCTACCTAACCGAAGCAGAGAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000042956:ENSDART00000063064:4
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0006717=p450=FE(11.7=100)
A:
NA
C2:
PF0006717=p450=FE(12.8=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCGCCCCTTTGACCCTTTG
R:
CTCTGCTTCGGTTAGGTAGGC
Band lengths:
334-1815
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]