DreINT0055701 @ danRer10
Intron Retention
Gene
ENSDARG00000035507 | ddx31
Description
DEAD (Asp-Glu-Ala-Asp) box polypeptide 31 [Source:ZFIN;Acc:ZDB-GENE-030131-3973]
Coordinates
chr5:29118373-29118749:+
Coord C1 exon
chr5:29118373-29118492
Coord A exon
chr5:29118493-29118642
Coord C2 exon
chr5:29118643-29118749
Length
150 bp
Sequences
Splice sites
5' ss Seq
CAGGTCAGT
5' ss Score
8.94
3' ss Seq
ATCTTGCTCGTCCATTAAAGGAT
3' ss Score
7.03
Exon sequences
Seq C1 exon
ACTGCGGAAGGGCATAAATGTTCTGATCTCGACTCCAGGCCGACTGGTGGACCACATCAAGAACACACTAAGTATTGCTTTCAGTGCTGTGCGCTGGCTCATTCTGGATGAAGCTGACAG
Seq A exon
GTCAGTGTGATTACTAATTTGATCAGATAATGTCATTGTGTGATGCACAAAGCTAAATACCTGTCTTATTCATAGTGAGATTTTCATTAATGACTATTTACATTTGTAGTATCATGTGTTATAACCAGTAATCTTGCTCGTCCATTAAAG
Seq C2 exon
GATTTTGGATTTGGGCTTTGAGAAAGATCTGACTGTGATTCTGAATGCACTGAACGCCGCTGGACCCGACAGGCAAAATGTGCTGCTTTCAGCTACTATAACTGAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000035507:ENSDART00000148314:10
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0027024=DEAD=FE(22.5=100)
A:
NA
C2:
PF0027024=DEAD=FE(20.2=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACTGCGGAAGGGCATAAATGT
R:
CACATTTTGCCTGTCGGGTCC
Band lengths:
202-352
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]