DreINT0059670 @ danRer10
Intron Retention
Gene
ENSDARG00000062083 | dph7
Description
diphthamide biosynthesis 7 [Source:ZFIN;Acc:ZDB-GENE-070410-57]
Coordinates
chr5:24576421-24576699:-
Coord C1 exon
chr5:24576627-24576699
Coord A exon
chr5:24576487-24576626
Coord C2 exon
chr5:24576421-24576486
Length
140 bp
Sequences
Splice sites
5' ss Seq
AAGGTCAGA
5' ss Score
6.8
3' ss Seq
ATACTGCTTTCCTGTCACAGGCA
3' ss Score
8.85
Exon sequences
Seq C1 exon
GTGGTGATGACAGCAAACTAAAGGGCTGGGATCTGAGAATGGGTTGCTCTTCCCCTACTTTCACCAGCAAAAG
Seq A exon
GTCAGACAGGAGTTAATATGATTTGTCATCCATCAACTTTACTTTTACCCTTTACCGAATCACTGTTTAAATGTTCTCTATGCCTGTTTAATGCTGTTTATGCACAATCGTTGTGAATAAATACTGCTTTCCTGTCACAG
Seq C2 exon
GCACACTATGGGGGTTTGCAGCATACACAGTAACCCTCATCGAGAGCACATACTGGCCACAGGAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000062083:ENSDART00000089339:6
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0040027=WD40=PU(20.0=32.0)
A:
NA
C2:
PF0040027=WD40=FE(55.0=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACAGCAAACTAAAGGGCTGGG
R:
CTTCCTGTGGCCAGTATGTGC
Band lengths:
130-270
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]