DreINT0065062 @ danRer10
Intron Retention
Gene
ENSDARG00000070839 | evi5b
Description
ecotropic viral integration site 5b [Source:ZFIN;Acc:ZDB-GENE-041111-244]
Coordinates
chr6:29045187-29047245:-
Coord C1 exon
chr6:29047105-29047245
Coord A exon
chr6:29045346-29047104
Coord C2 exon
chr6:29045187-29045345
Length
1759 bp
Sequences
Splice sites
5' ss Seq
CAGGTAAAT
5' ss Score
8.76
3' ss Seq
TTTGTGTTTATGCTATGCAGAGG
3' ss Score
5.13
Exon sequences
Seq C1 exon
AGAAATACATCCCTACCAGATGAGAATAATGTGGTACGTCTTCAGGAGGAGCTAATTGGTGTGAAACTGAGGGAGGCAGAGGCTTTAACAGGCCTTAAAGAGCTTCGACAGCAGGTCAGAGATCTGGAGGAGCACTGGCAG
Seq A exon
GTAAATACACACTCATAAATAACTTTATCATTCACAATTTGAAAATGTAATACGGACCAGTTGTGTCACAGCTAGGCATGGGACGATAATCGTTTTCAAGGTATACCGCGGTGTGGAAAAGTCAAGGTTTTAAAACAGTCAAAAATGTTCTGTAATACCGTTTCTAGGGTATGTGTAAGATTTTTATTTACATTTTTTTAGAACAACAATATCTTCGGCAGAAAATATATCTAAAGATGCACTTTTAAATAGTAAATAAATCTGTGTTTTTGAAACTAATGAAGACTAATGAAGAAGTCAATGATTCATTTGAATTATCTAGCCTGACATATTTACTGTTCCAAAACACTTAAAATCTTTCAAAAAATAAAATATATGGTTTTTAAAGGGGAAATGTTGTCAGACATTTAAAAAGAATACATTTTAAAGCAGTAATCACAATATCGTGGAACCGTGATATTTTTATCCAAGGTTATGATTTCGTCAGAGTTTTAAACCCCATGCCTAGTCACAGCTCAACTTGTGATTTCTTTTTCTGCTTTAATTTCAGTAAGTCTTTTCAACCGCTTCCACTGACATTCCATTAAAAAAGCTTCACATTTAAGATCTGCTTTCTTTTTAAAAAATCTTTACTGCCTGGTTGAGATATAATTTAAAGTGTCTCTCATACCAGACAAGAAGCACTGCATTACATTATTTTAAGCTGTCTTTTTAAAATATGGAAATTTGTTTTACCACAGTATTTTCAATGAAATGTCTGCGCTTGCCTTCTGCTCCTGACGGCGTTTGCATTTAAACACTCTTTCATCTAATTTTACTCTAAAACAACTTAGGCCCAATCGAAATTCTACCCCTTAGCCTACCCTTACCCCCTACCCTTTATTTTGTGTGTTTGCATGAAGATGTAGGGGTGTCCCAATTCTCTTTAGCTTGAAGGCATAGGGCTAAGGTGAAGGGGTAGATACTTCTTTGAACAGAGATTTTTCAGGAACACACACAAAACCAAGGAGTAAGAAAATTTCCCAGAATACACCAACCACAACGGCAGCGTAGCTACACCTGGAAGTAAGGAGATCCATGAACTAGTATTTTTTTGTCACTATTACGAGTTTTTACAACAAACAAGAATATGCTTTAATATATTCATAACCATGTTTATGTTTTACCGTAATGCTTTAAAAAAACGCTAAATAAAAACTGTAATATCTATAATCCCTAATAATAACCCTTGTATAGCAGTCCTACAACATATTGTCACTCAATGACGCTCGAATACCCTGTCAGAAAAGTCTAGTTATGATAACATGATGTATGCCTTCAGTGATTTCCTTAATACAAATACCAAAAAATAACACATCTGAAAAAACTACAGCAGTCTCGATCATCAATCTCATATGAAGCAAGAGATCACGATGACGTGTGCAGGTGCTGTAGTGATGTCCCATTTCATATGGGTAAAATTTGAAGCCCTTCCCTTTCACATTCTGTTTCAAGGGCCAAGAGGAAGGAGAAGGGGTAGGGGTACAAAAATAGAATTGGGATTCGGCCTAAATAAATGTTCTCTGGTCTATTTATCGAACTATATTTTAATTAAAATAGATTATCAATGCTGTAAAAGGAACCTTATGAAATTGAAAATATTCACATTAACCTTTCACTGGAAGTTTATTGGTGGCTGAAAAACACTTTGTGTTACAAAGCACTGTGCATATCAGCCATCAACCAATGTTTAAGTAAGATTTGTGTTTATGCTATGCAG
Seq C2 exon
AGGCATCTGGCACGGACGGCGGGCCGCTGGAAAGACAGTCCACGCAAGAATGCGGCAAGTGAGCTTCAGGATGAACTGATGAGTGTGAGGCTCAGAGAGGCAGAAGCACAAGCTGAGCTCAGAGAGACCAGACAGAGAATGCTGGAACTGGAGACTCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000070839:ENSDART00000124947:14
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.170 A=NA C2=0.698
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGAAATACATCCCTACCAGATGAGA
R:
CTGAGTCTCCAGTTCCAGCAT
Band lengths:
300-2059
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]