DreINT0078073 @ danRer10
Intron Retention
Gene
ENSDARG00000101482 | hk2
Description
hexokinase 2 [Source:ZFIN;Acc:ZDB-GENE-040426-2017]
Coordinates
chr5:13475442-13475843:+
Coord C1 exon
chr5:13475442-13475537
Coord A exon
chr5:13475538-13475743
Coord C2 exon
chr5:13475744-13475843
Length
206 bp
Sequences
Splice sites
5' ss Seq
GAGGTCAGA
5' ss Score
5.24
3' ss Seq
GTGTCTTTTATTTCTTAAAGGAG
3' ss Score
7.41
Exon sequences
Seq C1 exon
GGTATCCTGATAAAGTGGACAAAGGGCTTCAAGGCCTCGGGTTGTGAAGGAGAAGATGTGGCATCCTTGCTGAAAGATGCCATTCATCGCTGTGAG
Seq A exon
GTCAGAATGAGCGCTCGCCGCTGCTAGCACAGCATGGAAGCCATTTCTGCACTTATTAACCACAATTACCCAGCCTTTTATAACAAGATGTAATTAGTAGATGTTCAGTCAGGCGAATCGTGTCCCGAAAGGCAGTCATGGTGGATTTGCTTTGCTCTGGTTTGCTGGTAATACCTCCGTCTGTCTGTGTCTTTTATTTCTTAAAG
Seq C2 exon
GAGTTTGATTTGGACGTAGTGGCTGTGGTGAATGACACTGTGGGCACGATGATGACCTGTGGGTACGAGGACCCGCAATGTGAAGTTGGACTTATTGTGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000101482:ENSDART00000160690:13
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0034916=Hexokinase_1=FE(15.0=100)
A:
NA
C2:
PF0034916=Hexokinase_1=PD(11.2=67.6),PF0372711=Hexokinase_2=PU(3.3=23.5)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGTATCCTGATAAAGTGGACAAAGGG
R:
CCACAATAAGTCCAACTTCACATTGC
Band lengths:
196-402
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]