DreINT0080026 @ danRer10
Intron Retention
Gene
ENSDARG00000025375 | idh1
Description
isocitrate dehydrogenase 1 (NADP+), soluble [Source:ZFIN;Acc:ZDB-GENE-031006-1]
Coordinates
chr9:28312721-28314045:+
Coord C1 exon
chr9:28312721-28312898
Coord A exon
chr9:28312899-28313893
Coord C2 exon
chr9:28313894-28314045
Length
995 bp
Sequences
Splice sites
5' ss Seq
CAAGTGAGT
5' ss Score
9.1
3' ss Seq
CTTTTGAAACTGTGTTCCAGAGA
3' ss Score
6.38
Exon sequences
Seq C1 exon
GTACCGGTGGAGTAGCTCTGGGAATGTACAACACAGACAAGTCAATCCGGGACTTTGCTCACAGCTCTTTCCAGATGGGCCTGAATAAAGGCTGGCCAATGTACCTCAGCACCAAAAACACTATCCTGAAGAAATATGATGGGCGTTTCAAAGATATTTTCCAGGAGATCTATGACAA
Seq A exon
GTGAGTCGCTGGTTACATGGTTACTGAAAAAAGTCTCTGGTAATCGATGGAGACAATGGCTGTTTCTCAATATACATTCTATAAGTGATCTTATGTTCTTATGTAATGTCATGATCAACCACCAAAGGTCAATTCAAATATTCAAGAGCGCAAAACAAGGACGAGTGAAGGAAACCACGTGTTTTTATGGAGGATGCATAGAATGCAGTCAAGCCCAACATTTCATATTCAAGATATAACTTGTTTTTCTTAGGAGTTTTCCTAAATAAACACCTAAATAAACACTTGGAAATGTGTTTATTTGCTGAAATTCATATAAAAATCTGAATTGGCTGTACAGTATGTGCATTCAAAATGCTTTTTTACAGTTCAGTACAGTACAGGATAGATAGATAGATAGATAGATAGATGGATAGATGGATAGATAGATAGATGGATAGATGGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGAAAAAAGATAAGTCATTGTATGAAAGTTAATGTTTAAATTGTTTTCCGTTAAAACGCATAAAGGTCAAGTGACCATCAGAAAGATCACAGTGAGAAGCCAAGTCCTTTCTTTGCATTCTTGATTATGATAAAAAAATCCTTCCATTTTAAAACTAGTTTTAAAGGCTTACATATACAATATATATATATATATATATATATATATATATTCTTTACTAAATAGCATTTCCTTCTGTCTCCAGGGGTATGTCTAACCCGATTTCACAAAATATGTCATTATAGTCTTGAATTTTAAGATGAGGAATAGTAAACGCCTGTTTGTATTGAATAAAAAAAATAGAATCGCTCCTGCCTGTTGTTAATCAATTATGGCTGCACTGAATAAGCTGACAGAATAACAAACAATGAAGAAATAAATGTAGAAATATAACCTTTTGAAACTGTGTTCCAG
Seq C2 exon
AGAGTACAAGGCCAAGTATGAGGCCATGGGCATCTGGTATGAGCATCGGCTGATTGATGACATGGTGGCTCAGGCCATGAAGTCTGAAGGTGGGTTTATCTGGGCCTGCAAGAACTACGATGGAGACGTGCAGTCAGATTCTGTAGCTCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000025375:ENSDART00000007789:5
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0018015=Iso_dh=FE(14.9=100)
A:
NA
C2:
PF0018015=Iso_dh=FE(12.9=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTACCGGTGGAGTAGCTCTGG
R:
AGCTACAGAATCTGACTGCACG
Band lengths:
326-1321
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]