MmuINT0080178 @ mm10
Intron Retention
Gene
ENSMUSG00000025950 | Idh1
Description
isocitrate dehydrogenase 1 (NADP+), soluble [Source:MGI Symbol;Acc:MGI:96413]
Coordinates
chr1:65165100-65166284:-
Coord C1 exon
chr1:65166107-65166284
Coord A exon
chr1:65165252-65166106
Coord C2 exon
chr1:65165100-65165251
Length
855 bp
Sequences
Splice sites
5' ss Seq
CAAGTAACT
5' ss Score
4.45
3' ss Seq
TGTTCGTTTGCTTGTTTTAGGAA
3' ss Score
10.03
Exon sequences
Seq C1 exon
AAGGTGGTGGTGTTGCCATGGGCATGTACAACCAGGATAAGTCAATTGAAGACTTTGCACACAGTTCCTTCCAAATGGCTCTGTCCAAGGGCTGGCCTTTGTATCTCAGCACCAAGAACACTATTCTGAAGAAGTATGATGGGCGTTTCAAAGACATCTTCCAGGAGATCTATGACAA
Seq A exon
GTAACTATGGCCCTTACTCCCTTTTCTGTTTTCAAGTAAGAATTGCAGGCATTTAGGATGTAGCCCCTGGGGCAGACTGAATGAGCCACAGCACTGCCCTGAAATTGGGTAATGGCAAATCTGAGATTTAGCAGGGCTGAAAGGAAACTACTCTGAGGTGCTCTTAGAAGGTAAATGTGTTCCTAAATAAGGGCCATGTGTTTTCTTTTCTTGGTAGGGACAGGGCTATTCAGTGTTTGAATAAAGTAGCCCATTTATGTTGAAAAGTGCCAATGTTTTGAAAGTGTGTGTTTCTGAAGCCCGCCCAGTTTATTTTTCTTAAGACCTTTGCTTGAATTGAAAGGTCTGCTTTAAGAGGTTCTGTTATTTGTATGTAACAGTTCTCATTTCGGCCACACAGGAGGCATGCTTGGGTCCGCTCCTAACATGGCATAACTCCCAGTTAGGGCAAGTGTTCAGATGGGAGCTGAGAAATGAAAGGAGACCTTTGCTGAAGTTTGGAGAGTCCTCTTCCTATGGCCCATCTGTTCCAAGCTTGGGCACTGTGACATTTTTAAAATAAATTGAATCACAAAATCTGATCATTATTAAAAATGTAGGAAGCAAAGAAATATGTGGGGGAATCTGTTTTTTCTCGCATAATTCTTAGACCTTCATTCGTAAGAGAGAATAGTCTTATGAGAGATGGCCACTTTGAGGGTCTAGGATAGGAATCTAAGCATTTGTCTGTCCCAAATTTGTGCTGTATCTACTAACAATAATGTTTCCTGTCCTTTTGGAGAGTTGCAAATGGTATCTCTGATAAAACTAATTCTATTTTATTCTTGTTTTCTCTTGTTCGTTTGCTTGTTTTAG
Seq C2 exon
GAAATACAAGTCCCAGTTTGAAGCTCAGAAGATCTGCTATGAACACAGGCTCATAGATGACATGGTGGCCCAAGCTATGAAGTCCGAGGGAGGCTTCATCTGGGCCTGTAAGAATTACGATGGGGATGTGCAGTCAGACTCAGTCGCCCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000025950:ENSMUST00000097709:5
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0018015=Iso_dh=FE(15.0=100)
A:
NA
C2:
PF0018015=Iso_dh=FE(13.0=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AAGGTGGTGGTGTTGCCA
R:
CTTGGGCGACTGAGTCTGACT
Band lengths:
330-1185
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types