DreINT0091810 @ danRer10
Intron Retention
Gene
ENSDARG00000028533 | macf1a
Description
microtubule-actin crosslinking factor 1a [Source:ZFIN;Acc:ZDB-GENE-030131-3606]
Coordinates
chr19:35568499-35570369:-
Coord C1 exon
chr19:35570286-35570369
Coord A exon
chr19:35568607-35570285
Coord C2 exon
chr19:35568499-35568606
Length
1679 bp
Sequences
Splice sites
5' ss Seq
AAGGTACAG
5' ss Score
8.04
3' ss Seq
TCTTCCTGTTTATGCATTAGGAC
3' ss Score
7.35
Exon sequences
Seq C1 exon
GCATTGGTAGTTGAGATTGAACAGAACCAGGTCAAACTGGATGAGTGCCAGACCTGCTCTAAACAGTACAGCGCTGCCGTTAAG
Seq A exon
GTACAGTAAGCAATACCTTAAACTAACCAGTGTTGACTACTAGATAAAGTCAAAAGTATATCTCTTTTATGCGCATGTAAGAATAAAAGCAAAATATCTAGAGTTTACATTGTGGTAGTCAACAGATATGTTTTAGGGAGGAGTATTTTATCTATTTATTTATTTATTTATTTATTTATTTATTTGTATTTATTTTTTCAAGTTGGTGTCTGCAGTGTGATAAAACGTCTTCAATTTTGAAAACTAAAGATAAAAGTACATTAAAATATTGTGTTTTAGGTCTTAAATAATTTTAAACAGGTCTTCATTTTAGAATTAGGCCGACACTAATTCACTTACCAACAACCCATCTCAATAAAACTTTTATCTAACGTTAAATTTGTTAACATGTTGTTTTTTAAGTGTGTGTGTATACAACTAGAGTGTGCATGACAATTAAATATTTTCCACTGGTCCAACTGGAAAAATGTCTTAGTGATAAGCCCTGTATAAGTCTGAAATTTCATTCCTAATGAGCTTGAAAAGTCATAAAAATTCATAAATTTGACTTGGTGAAAACTGCAGAAAACCTGTGAAAAGAAAAGATTTGTAAATGATTCCTCCTAGCATGGTTCAATTGTCAGAACATTACACAGATTATATTTATTAACTTAAGAAAGGGCTCACTGCAAAAATGCAGTTTCGTATACAAAATATGTACTCACCAGACACTTTATTAGGTACATCTTACTAGTACACTTTTGCCTTCAGAACTGCCTTAATTGTTTGTGACATAGATTGAACAAGGTACTGGAAATATTCCTCAAGATTTTAGTCCATATTGACTTGATAGCTTTACGCAGTTGCTGCAGATTTGTCGCCTGCACATCCATGATGCGAATCTCCCATTCCACCACATCCCAAAGGTGCTCTATTGGATTGAGATCTGGTGACTGTGCAGGCCATTTGAGTACAGTGAACTTATTGTCATGTTCAAGAAACCAGTCGTAGTAGATCAATCTAAAAATACTCTTACCAGCTTGTCTGACACCAACAACCATGCCACGTTCAGTCTCTTAAATCACCTTTCTTCTGCATTCTGATGCTCGGTTTGAACTGCAGCAGACTGTCTTGACCATGTCTGCATGCCTAAATGCATTGAGTTGCTGCAATGTGATTGGCTGATTACAAATATGCGTTAGCAGTTGGACCTAATAAAGTGGCCAGTGAGTGTAAATCTAATATGCACCTATTACAAAATGTGCTTCATTATTATTATATGCAAACACAGCCTCTCAACACAAAGATCCATCCTTGTCTAATTATGTTTTTCTTCTGTTTTGTGGTTATCTTTTATTTCTCTCATACGTACACATCTCTTTTTCCCTATCTCCAGTACTTTTCTTGTTCGGTCTTTTTTTTCTTACTGTGAAACTATAGCCGGGTTCAATGTTGCCCCCTTGTGGATAAACAAGAAAACCTCAAAGCATGTGTGCAACCTTGTACATGGTATAATACGCATGTGCAGTGCACATTTAATGGGCTGATTGTATAATATACTTGCAAAATCCTCTACAGTATGTGTGAAAGTATACTTTGGGATTTTTATTTCCAATCATTTCGAAGAAATGCAAACAACCACTTACTTCACAAAATATAAATGCTAAAATGTTTACCGTGTCTTCCTGTTTATGCATTAG
Seq C2 exon
GACTATGAACTTCAACTTATGACGTTCAGAGCATTTGTTGAATCAACCCAGAAGTCCCCTGTGAAAAGGAGGAGGATGCACTCTTCCTCAGATGTCATCACACAGGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000028533:ENSDART00000146394:29
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.277
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
PF086146=ATG16=PU(3.5=17.1)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCATTGGTAGTTGAGATTGAACAGA
R:
TCCTGTGTGATGACATCTGAGGA
Band lengths:
191-1870
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]