RnoINT0088154 @ rn6
Intron Retention
Gene
ENSRNOG00000016047 | Macf1
Description
microtubule-actin crosslinking factor 1 [Source:RGD Symbol;Acc:1306057]
Coordinates
chr5:141171182-141171872:-
Coord C1 exon
chr5:141171789-141171872
Coord A exon
chr5:141171290-141171788
Coord C2 exon
chr5:141171182-141171289
Length
499 bp
Sequences
Splice sites
5' ss Seq
AAGGTAATT
5' ss Score
8.83
3' ss Seq
CAAATTTCTCCTGTTCTTAGGAC
3' ss Score
9.15
Exon sequences
Seq C1 exon
GAACTGTTTGCTGAGATTGAGAGAAATCAGACAAAACTGGACCAATGTCAAAAACTTTCCCAGCAGTACTCCACTACTGTAAAG
Seq A exon
GTAATTGTACCATATCCCGGAAGGAATAAATGATCTTCAGGTTAGGACTGATACATTATCAGCCTAAGTTCTTTTCTGGATAGATTATACAGTGCTCTCCTAGAGGTTGTCTCTGGCTTCAGTTTTTACTGTCACATAAGACTGAACACGGTGGTGCACACCTAATGCCAGCATTTGGAAGGGTTAGGCAGGAGGATCAGATGTTCAAGGCCTTCCCCACTTCCCCCACTGGGAGTTTGAGACCACCCTAGGCTACATGAGACCCTAGCCACTAAATAAGTAAATAAGAACTTGAATTTTCAGAGTAAGTAAAACTATATTATAAATAGCTATATTCATTTGTAGAGACGACACCAGTATTTCTTGCAGGCAGAATGGCCCAATGTTTGTGAACAAACACTAAATGTAGTCCAGCAAGAAAATACTATGTTTTCGTTATGTGCTGTGTTGTTAGCTAAGAAGTCTTAAGTAAAAGTAGTCAAATTTCTCCTGTTCTTAG
Seq C2 exon
GACTATGAGTTGCAGCTTATGACGTACAAGGCCTTCGTGGAGTCACAGCAGAAATCTCCGGGCAAGCGCCGTCGCATGATTTCCTCTTCAGATGCCATCACACAGGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000016047:ENSRNOT00000045400:29
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.278
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACTGTTTGCTGAGATTGAGAGA
R:
CTCCTGTGTGATGGCATCTGA
Band lengths:
190-689
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]