DreINT0091846 @ danRer10
Intron Retention
Gene
ENSDARG00000028533 | macf1a
Description
microtubule-actin crosslinking factor 1a [Source:ZFIN;Acc:ZDB-GENE-030131-3606]
Coordinates
chr19:35495015-35495902:-
Coord C1 exon
chr19:35495743-35495902
Coord A exon
chr19:35495342-35495742
Coord C2 exon
chr19:35495015-35495341
Length
401 bp
Sequences
Splice sites
5' ss Seq
AAGGTAGAG
5' ss Score
6.38
3' ss Seq
ACTCTGGGCTACTATCCCAGGCT
3' ss Score
3.45
Exon sequences
Seq C1 exon
GCAGAAGCAGCTCGAGGAACTGCAGGTGCTGGCATTACAGTTCCATGAGGCCGTGGAGCCGCTCAGTGAGTGGCTGAGCGCAACAGAGAGACGCCTGAGCTCTGCTGAGCCAATGGGAACCCAGACATCCAAAATCACACAGCAGATTGTAAGGCACAAG
Seq A exon
GTAGAGTATACACAGCAGAAGATAGACAGAACCGAGATCTGTCATTTTAGCAAGCTACTTTCAGTGTCGAGGATCATTAGACTGACTAGAAATGCAGAGGAAGAGAACAGATTGTGAATCTCCTGTGGTTTTAAATGAACCAGTAGGAGTGTGGTTATCTAAGAAGACATGAAATTCAGGTCTCTGGTCGTGATCCTGCCCACATACATCTCCATTTCATTATGGTTTCATTTTTTATGATTGTGTTTCATTTCATTTTGTTTTGATCCATGTATTTTTTTGTTAATTATGATTTGTCGTTATTCTGCATTGTCATATATGTTTATGTTTGCCTTGTCTTTTTTTTTCCTTCTTCTTATCATCCAATCGCATCTTTTCTTTACTCTGGGCTACTATCCCAG
Seq C2 exon
GCTCTCCAAGAGGAAGTCTCCTCTCGAGAGAATAATGTAGACCGTCTTGAGAGTCTCAGCCAATCGCTCCATCCGCTCAGCTGCGCAGCCGATCAGGATTGGCTGGGCGAGCGTGTGGGTGCAGTGCGCAGCGGGCACACAGAGCTGCGTGATTGGTGCGTTCGGAGAGAGGCCATGCTGGAGCAGGCGCTGGCCAATGCACAGCTGTTTGGAGAGGAAGAGGTGGAGGTCCTGAACTGGCTAGCTGAGGTGGCCCAGAGACTGGCTGAGGTCTCAGTGCAGAGTTATCAGCCGGAGCACCTAGAGCAGCAGCACAAACACACACTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000028533:ENSDART00000146394:61
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.333 A=NA C2=0.147
Domain overlap (PFAM):
C1:
PF0043516=Spectrin=PD(5.6=11.1),PF0043516=Spectrin=PU(41.1=81.5)
A:
NA
C2:
PF0043516=Spectrin=PD(57.0=56.0),PF0043516=Spectrin=PU(41.1=40.4)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGTGCTGGCATTACAGTTCCA
R:
CCTCTCCAAACAGCTGTGCAT
Band lengths:
353-754
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]