DreINT0094981 @ danRer10
Intron Retention
Gene
ENSDARG00000017229 | megf10
Description
multiple EGF-like-domains 10 [Source:ZFIN;Acc:ZDB-GENE-080506-1]
Coordinates
chr10:16188567-16188935:+
Coord C1 exon
chr10:16188567-16188695
Coord A exon
chr10:16188696-16188806
Coord C2 exon
chr10:16188807-16188935
Length
111 bp
Sequences
Splice sites
5' ss Seq
AAAGTAAGT
5' ss Score
9.72
3' ss Seq
CCTGTCTTCTTGTCCTGCAGGAT
3' ss Score
12.67
Exon sequences
Seq C1 exon
CATGTCCTCCGGGTCAGTGGGGTCCCAACTGCATTCACACTTGCAACTGTCACAATGGAGCATTCTGCAGTGCTTATGACGGCGAGTGTAAATGTACGGCCGGCTGGACAGGTCTCTACTGCACACAAA
Seq A exon
GTAAGTACCAAGTTCTTTTGCAATGATTCCCTTTCAATTACTAGCAGCTGTAATTGGTTAGGAAGGTCGATAACATTTGGTCATGGCATTTCCTGTCTTCTTGTCCTGCAG
Seq C2 exon
GATGTCCATTGGGTTTCTATGGTAAGGATTGTGTGCAGGCATGTCAATGTGAGAATGGTGCAGACTGCAATCACATCTCGGGTCAGTGCACATGCCGTACAGGCTTCATGGGACGTCACTGTGAAACGA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000017229:ENSDART00000043936:17
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0005319=Laminin_EGF=PD(28.6=27.3)
A:
NA
C2:
PF0005319=Laminin_EGF=PU(63.0=65.9)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATGTCCTCCGGGTCAGTGG
R:
CGTTTCACAGTGACGTCCCAT
Band lengths:
256-367
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]