DreINT0094994 @ danRer10
Intron Retention
Gene
ENSDARG00000017229 | megf10
Description
multiple EGF-like-domains 10 [Source:ZFIN;Acc:ZDB-GENE-080506-1]
Coordinates
chr10:16151237-16151668:+
Coord C1 exon
chr10:16151237-16151354
Coord A exon
chr10:16151355-16151531
Coord C2 exon
chr10:16151532-16151668
Length
177 bp
Sequences
Splice sites
5' ss Seq
TGGATGGTA
5' ss Score
-15.52
3' ss Seq
TGTATTGATGTTACTTTCAGGGA
3' ss Score
7.29
Exon sequences
Seq C1 exon
CTGTGAGGACTTGTGCCCTCCGGGAAAACATGGACAGCAATGTGAGGAGCGTTGTCCCTGTCAGAACGGTGGTGTGTGTCATCATGTGACTGGAGAATGTTCCTGTCCTGCTGGTTGG
Seq A exon
ATGGTACGCCTGGCTGTTACAGTTCTGAATGTGGCTCTCGTAAATATTAAAACTGAAATTTATAAGTTATATAGTTTCATCATGTTTGGCACTATTATTGCAGTCATTATGAAATGTCTTTGCATTGGAGGAAACATTCCATTATCCATTATGTCAGTGTATTGATGTTACTTTCAG
Seq C2 exon
GGAATGGTGTGTGGACAACCCTGTCCGACGGGACGCTTTGGTAAGAACTGCTCACAGGAGTGTCAGTGTCATAATGGAGGAATCTGTTCGCCATCCACAGGACAGTGTGTGTGCAGTTCAGGATACACAGGAGAGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000017229:ENSDART00000043936:7
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF126612=hEGF=PD(7.7=2.5)
A:
NA
C2:
PF079748=EGF_2=PU(93.5=63.0)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Rat
(rn6)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTGTGAGGACTTGTGCCCTCC
R:
ATCCTGAACTGCACACACACTG
Band lengths:
242-419
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]