GgaINT1017736 @ galGal4
Intron Retention
Gene
ENSGALG00000014699 | MEGF10
Description
multiple EGF-like-domains 10 [Source:HGNC Symbol;Acc:HGNC:29634]
Coordinates
chrZ:56224369-56226354:+
Coord C1 exon
chrZ:56224369-56224489
Coord A exon
chrZ:56224490-56226217
Coord C2 exon
chrZ:56226218-56226354
Length
1728 bp
Sequences
Splice sites
5' ss Seq
ATGGTAAAA
5' ss Score
4.79
3' ss Seq
TTTTTTTTCTGCATACACAGGGC
3' ss Score
7.88
Exon sequences
Seq C1 exon
CTGTGAGGATCTCTGCCCCCCGGGGAAGCACGGGCCGCAGTGTGAGGAGAGATGCCCCTGCCAGAATGGAGGCATCTGCCACCACGTGACCGGGGAGTGTGCATGCCCACCAGGATGGATG
Seq A exon
GTAAAAACCCTTTCTAAAATCAACAGTGCTCTGCAAAATTTCCATATTTCTAGATTTCAGGCAGCATCAGAAAAGAGCTGTGGATTAGAACATAAAATGCAGTCCGCCTGTAGCCAGGAGTAATAAGAAGTGTATTTCAGCATAAAGAACTGGAATAAAATACTTGAAAAACCATCTGTTGCTGAAAATCCTCCAGTTCTGCACTGCTGTTTTAACCATGTAAGGTCCTGGGAAGCAAGGGATGTGAATTATCCTTTGAATTTTGTCTAGAAAATGTTCACTTTGAGATAGAACATAATACTCTAGGGAGCCGATACCTCCTTAGAAAGACATTATGTATTTTTCGCAGGGTATTTTGCATCTATTTTCAAATGTATAGTATGCATTAAAAAAAAAAAAAAACAACACCCAAATTTTGAGGTATGGTGTTAAGTGATGCTAACAGAAACAAGACATCAGAACTTGACCATTGTCTTTTGACTTTCAACCCTCTGGTCAGTGGATAGGGAAAGATGCATACAAATGAAATTCCTTGTTTGCATGCAGGCTCCCTTACCAGACTGCCACTTCTGAGTTAGGAAGTTGGAAGGTGATCAAAAGGATGGAAAGTGTTTATTGAACTTCTAATTATCTCATGATATATGAGGAGGTCAGTGGTAACATGAGCTCTTCACCAGCTAGGGTACATCACTGTGAGCAAATGATGTCTAACTATGTAAGCACTGCTCACCTGTCAACCTACCTTGGATTATAGTAATTTTAACATTTATTCCATTTCGTGATGGAAGAACATAAATTACTGATCACAGGATACAGTTATTTGCATTTTGTCTTTAATTCCTCTACTGGCATCAATGCCACTAATGTAAAGACATACAGCATATGAAGAAATAGTGAAAATTTTGTGCCAGTGTGTATCTTAACTAATAAGTTACTATTTAGCTTTCAGATTAGTTATCCATTCCAAATAGCTATTTAAGCATAATTCAATGTTAGTTTTAGATTTTTCAATTTTGTGTGAAGCAATTTTGTTAACATAACCTTTTTTGCTTTCCATGTAAAGTTGGCGTAAAGTAGTCAAATACAAGCTCTAAAGTGTTTTATGGTAGCTAAAATTCCCATGTATCTTTTGCCTGATGAGATACCTATAAATCTGCTTCTCTTGAATTGGCTTCTTAAAAGTCTTAAGGTCTGATATTTTTGTCAGTACATAACTGCAGTTAATTCCAAATATGTAATTAGCAGTGCAGAAATTGAAATTTTGTGGAAAAGAAGCTTTTAAGGTATTTCCCTGCTTTCAGTGCAATTAGGCAGCTTTTCCTTTCATTGGAAATAACAAGAGATCACAAAATCATGTAATTGAGGCATTACAGCAGTTGTAGATTTCTATGCTCTTAATTGCTGGGGGGCATTTCCAGATGTCTTGATATATATGACTGCTAATAAAGAAATTGGGAAGAGTTTGACATCAATAAGTCTTAAATGTTTTTTAATTAATTGTTGCTAAGAATTAGTATTTTCCATGAGTTTTGGCCTGCTAAATGGAAATCCTGGAGGACTTTACTATTTAATGCTGAAATATAGACTGTGAGATGGAGATGAGCCTTTCATTTCCAAGATCTGCACTAGATGAGTAACATGATCTTGATCTCCCTGCATTACAAACCTTTCTTCCTTGTCCATTCAACCTGAAATCAAAATGTAAGACTTTTTTTTCTGCATACACAG
Seq C2 exon
GGCATGGTCTGTGGTCAGCCTTGTCCTGAAGGTCGTTATGGGAATAACTGTTCCCAGGAGTGTCAGTGCCACAATGGAGGGATCTGCGACTCAGCGACAGGTCAATGCCATTGCAGCCCAGGCTATACAGGAGAACG
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000014699:ENSGALT00000023694:7
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF126612=hEGF=PD(7.7=2.4)
A:
NA
C2:
PF0005319=Laminin_EGF=PU(54.3=54.3)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTGTGAGGATCTCTGCCCCC
R:
GTTCTCCTGTATAGCCTGGGC
Band lengths:
257-1985
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]