DreINT0095029 @ danRer10
Intron Retention
Gene
ENSDARG00000079199 | megf6a
Description
multiple EGF-like-domains 6a [Source:ZFIN;Acc:ZDB-GENE-090312-12]
Coordinates
chr23:21212236-21212610:-
Coord C1 exon
chr23:21212482-21212610
Coord A exon
chr23:21212365-21212481
Coord C2 exon
chr23:21212236-21212364
Length
117 bp
Sequences
Splice sites
5' ss Seq
TGGGTAAAG
5' ss Score
4.2
3' ss Seq
ATCTTCTGTTGTGTGTTCAGAAT
3' ss Score
8.64
Exon sequences
Seq C1 exon
AGTGCTCAGCTGGATTGTATGGTGTGGGCTGCAACCAGCATTGCCTGTGTCAGAATGGAGGGTCGTGTGACCGAATCAGTGGCCACTGCACATGTGTCAGTGGGTGGACAGGAGTGGCCTGTGAATTGG
Seq A exon
GTAAAGCACAGACCCAACACACTGTAGTAACTCCAACAGCTGCTGGCAATCTTAAGATAAAACCTTTTGACAGATGCAAAGGTCACAGATCTCATTTATCTTCTGTTGTGTGTTCAG
Seq C2 exon
AATGTGCTGCGGGACACTTTGGCATGGACTGTCAGCACAAGTGTGAGTGTGTAAATGGCGGCGTGTGTGACCGTCACAGTGGCAGGTGTTTGTGCCCAGCAGGTTGGACAGGCACGCACTGTGAGACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000079199:ENSDART00000143206:22
Average complexity
IR
Mappability confidence:
NA
Protein Impact
Alternative protein isoforms
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
PF126612=hEGF=WD(100=29.5)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GATTGTATGGTGTGGGCTGCA
R:
CTCACAGTGCGTGCCTGTC
Band lengths:
242-359
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]