DreINT0095462 @ danRer10
Intron Retention
Gene
ENSDARG00000015349 | mfge8a
Description
milk fat globule-EGF factor 8 protein a [Source:ZFIN;Acc:ZDB-GENE-050327-9]
Coordinates
chr7:48515450-48516952:-
Coord C1 exon
chr7:48516797-48516952
Coord A exon
chr7:48516263-48516796
Coord C2 exon
chr7:48515450-48516262
Length
534 bp
Sequences
Splice sites
5' ss Seq
AAGGTTAGA
5' ss Score
6.12
3' ss Seq
TTTGTTTTTATTCCTTGCAGATT
3' ss Score
11.29
Exon sequences
Seq C1 exon
CTGGATTTGGAGAAACCTGTAAGGGTCACAGGCATCATCACTCAGGGAGCAAAAGACTTTGGAGTTGTGCAGTTTGTTTCGGCCTTTAAGATCGCTTACAGTGATGATGGAAAGTCCTGGAGCATTGTGAAGGATCAAGTAACAAAAACAGATAAG
Seq A exon
GTTAGAGCAATGACGTTATCTTTATTCAATAAATAAATAGATTAGATTACATTAATTAAAACTGTGTTTGAAGAATATGGAATACTAGATTTAGAACTAGCTAGAATATATTTGCTTATTAACATCGTAAAGATGAATGACTCTGTCACCGTAAGTAATATGAATTCAATATCAATATTAATTAAATGTTAATAACTTATTACATATTCAGAAAGGGAAAGGTGACCTAAAAATGAAAATTTAGTCATCTTTTACTCACCATTTACTTGTTTCAAACCTTTATGAGTTTTTTATGTTAAACACAAGAGAAGACATTTTGAAGATAGCTGAAAACCTGTAACCATTGACTTGCAACATCTGATCGTCAGTTCAAGCAGATGCAAGCTCTATTTAATACATTTCATTAGCTAAATATATTTGTAAAAACTGTAAGAACAACAACAAAAAGGCATGCACAGGATGAGCCCTACAATACATGCCTGATACTCATGTCACCTTACTTACTGAAATTAGTTTTGTTTTTATTCCTTGCAG
Seq C2 exon
ATTTTCCAAGGCAACACTGACAACAACACGCACAAAAAGAACTTGTTCGAGCCTCCGTTCTATGCCCGTTATGTACGCATTCTGCCATGGGAGTGGCACGAGCGGATCACCCTGCGCATGGAGCTGCTGGGCTGTGATGATTAGTCAACATGACACTCTTATTCACTATCTGAAGTGTGTCTGCTGGGTGATTTTCCATTTACATTAGCCTCCGTTCTATTGCTCTGTATGTTCTTGTACTGATGCAATTACTTGAAGGGCTCAGTTGATCTAATTTTGAAAGCCTTTGGCCACAGCGGTGAGTTTCTGAAATGACGTTTTAAGTACATTTCTGAGTATTTTACAGAATTGAAAGAAAACACAATGAGACTAATTTTAATGTTTTACTAAAATCTTTAATCTTATAAACACTATTTCGAAATGTACACACTCGTTAATTTCTTTTAGTGTTATTGCTTATCCTTCGTCACTAAGGTGAGTAAAAACCTTCAAGTGTTACATTTTGCAGCCCATTAGTGCTGATGTTCATCAGTTTACCTTATTTTGCCTTTCAGCACATGTACAGGATTGATTTGTAACGGAAAGTTCAACTTGTAATTCTGAGTTTCTGCTTTTTGTACGCACAGCTTGAATCAGATCTGTCAGAAACCGTGCTGTAAATCAGAATTGAATTGAAAACTTTTATAATCAATGAGCTTGTTGAGAAAGCTGCTCCAATCAATTGACCTGTTTGCACTGCCATTGGTATACACTTTTATAACGCATTTTATAAATTTGGCCAACGTAAACAACCGAATTAAAAAAAGAAATGTA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000015349:ENSDART00000139400:9
Average complexity
IR
Mappability confidence:
NA
Protein Impact
Alternative protein isoforms
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.072
Domain overlap (PFAM):
C1:
PF0075420=F5_F8_type_C=FE(36.4=100)
A:
NA
C2:
PF0075420=F5_F8_type_C=PD(29.3=85.4)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACAGGCATCATCACTCAGGGA
R:
ACAGAGCAATAGAACGGAGGC
Band lengths:
357-891
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]