DreINT0099045 @ danRer10
Intron Retention
Gene
ENSDARG00000070331 | muc5.1
Description
mucin 5.1, oligomeric mucus/gel-forming [Source:ZFIN;Acc:ZDB-GENE-120822-1]
Coordinates
chr25:8295383-8295764:+
Coord C1 exon
chr25:8295383-8295521
Coord A exon
chr25:8295522-8295656
Coord C2 exon
chr25:8295657-8295764
Length
135 bp
Sequences
Splice sites
5' ss Seq
CACGTATGT
5' ss Score
7.26
3' ss Seq
GGACTGTGTAACTTCTTTAGGAT
3' ss Score
7.32
Exon sequences
Seq C1 exon
CACATGCACAAACGGTATGTGGATTTGTACACAGAAGGCATGTTACGGCACCTGTACCATCTACGGTGAAGGTCATTTCAGGACTTTTGATGGCAACAGATACTCCTTCCATGGAGACTGTGAACACACCTTAGCCCAC
Seq A exon
GTATGTTCACTTTTGTATCTGCCAAGGCATTAAAATCTAAAATGAATATCCTACAATTGGTTAAAAGTAATTCTGTGTTGACTTGTTTCATGCTATTCATAGTGATTTGTAAACTGGACTGTGTAACTTCTTTAG
Seq C2 exon
GATTATTGTGATGGAAACCCCAGTCCCTCTTTCCGCCTTGTCACCGAAAACATCCCCTGTGCAACAGCCAACTCCATTTGCTCCAAGTCTATTAATCTTTTCTTCGGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000070331:ENSDART00000153708:22
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0009313=VWC=PD(65.6=85.1),PF053758=Pacifastin_I=PD(55.9=40.4),PF0009420=VWD=PU(18.8=59.6)
A:
NA
C2:
PF0009420=VWD=FE(23.5=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACATGCACAAACGGTATGTGGA
R:
CCCGAAGAAAAGATTAATAGACTTGG
Band lengths:
246-381
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]