DreINT0099807 @ danRer10
Intron Retention
Gene
ENSDARG00000100972 | myh11b
Description
myosin, heavy chain 11b, smooth muscle [Source:ZFIN;Acc:ZDB-GENE-101124-2]
Coordinates
chr3:36550950-36551389:-
Coord C1 exon
chr3:36551258-36551389
Coord A exon
chr3:36551157-36551257
Coord C2 exon
chr3:36550950-36551156
Length
101 bp
Sequences
Splice sites
5' ss Seq
CAGGTAAAG
5' ss Score
9.65
3' ss Seq
CTGAAGCATTTACTCAACAGGTT
3' ss Score
4.64
Exon sequences
Seq C1 exon
GTCAAACCCCTGCTTCAGGTGACTCGTCAAGAAGAGGAGATGAATCAAATAGAAGAGGAGCTCCAGAAAGCCAAAGAAATTGCCCAGAAGTCTGAAACGGAACTAAAGGAGATCACACAGAAACACGACCAG
Seq A exon
GTAAAGTCAGATCAAAAATGTGACACAAACTCAGCCGTAATTACTATTGTCATTCATACAGATTAAATATGCACTTACCAGCTGAAGCATTTACTCAACAG
Seq C2 exon
GTTGTGGAGGAGAGGAATAAGCTGCAGGCGAAGCTCCAGGAGGAAGCAGAACTGTACGCAGAGTCTGAGGAGGTCAGGATACGACTGGAGACCAAGAAGCAAGAACTGGAGGAGGTGCTGCATGAGATGGAGGCTCGACTTGAGGAAGAGGAGGAGCGCAGTCAGGCTTTTCAGCAAGAGAAAGGGAATTTGCAACAAAAACTTAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000100972:ENSDART00000166896:19
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.364 A=NA C2=0.522
Domain overlap (PFAM):
C1:
PF0157614=Myosin_tail_1=PU(13.0=79.5)
A:
NA
C2:
PF0157614=Myosin_tail_1=FE(25.3=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Rat
(rn6)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGAGGAGCTCCAGAAAGCCAA
R:
CTTGCTGAAAAGCCTGACTGC
Band lengths:
257-358
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]