DreINT0099815 @ danRer10
Intron Retention
Gene
ENSDARG00000100972 | myh11b
Description
myosin, heavy chain 11b, smooth muscle [Source:ZFIN;Acc:ZDB-GENE-101124-2]
Coordinates
chr3:36547833-36548254:-
Coord C1 exon
chr3:36548048-36548254
Coord A exon
chr3:36547938-36548047
Coord C2 exon
chr3:36547833-36547937
Length
110 bp
Sequences
Splice sites
5' ss Seq
AATGTGAGT
5' ss Score
7.8
3' ss Seq
TCTTTCGTGAACTCTTTTAGACA
3' ss Score
5.72
Exon sequences
Seq C1 exon
GCCAAAGCCACTCTGGAGAAGGCTAAACAAGCCCTTGAGAAGGAGGTTGGAGACCTAAATGGAAACCTTCGCTCACTGGGTAATGCCAAGCAGGATTTGGAGCAGAAGAAGAAGAAGGTAGAGACCCAGCTTGCTGACCTTCAGACTCGTTTCAATGAGAGCGAGAGAAAAAGGGAGGAGCTTGGAGATGCAGTGTCCAAACTTAAT
Seq A exon
GTGAGTTTTACATTCAGATTAATTTACTGAGTAAACAATATGGTATAAACGAAACAATATTTCTGTACAGGCATATTCGAATAACGAATGTCTTTCGTGAACTCTTTTAG
Seq C2 exon
ACAGAGTATAACAATGTGAACAGCATCCTCAATGAGGCAGAGAGCAAGAACATCAAGCTGAGTAAAGACGTTGTCAGCCTTAACTCACAGCTCCAAGATGCACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000100972:ENSDART00000166896:26
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.957 A=NA C2=0.457
Domain overlap (PFAM):
C1:
PF0157614=Myosin_tail_1=FE(8.0=100)
A:
NA
C2:
PF0157614=Myosin_tail_1=FE(4.0=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Cow
(bosTau6)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTTCGCTCACTGGGTAATGCC
R:
GTGCATCTTGGAGCTGTGAGT
Band lengths:
244-354
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]