MmuINT0103274 @ mm9
Intron Retention
Gene
ENSMUSG00000018830 | Myh11
Description
myosin, heavy polypeptide 11, smooth muscle [Source:MGI Symbol;Acc:MGI:102643]
Coordinates
chr16:14208325-14209810:-
Coord C1 exon
chr16:14209604-14209810
Coord A exon
chr16:14208430-14209603
Coord C2 exon
chr16:14208325-14208429
Length
1174 bp
Sequences
Splice sites
5' ss Seq
CAGGTGAGG
5' ss Score
10.07
3' ss Seq
CTCTTCCCCATCCCCTCCAGAAT
3' ss Score
9.22
Exon sequences
Seq C1 exon
GCCAAGGCAAACCTGGACAAAAGCAAGCAGACACTGGAGAAGGAGAACGCGGACCTGGCTGGGGAGCTGCGTGTCCTGGGCCAGGCGAAGCAGGAGGTGGAACACAAGAAGAAGAAGCTGGAGGTGCAGCTGCAGGATCTGCAGTCCAAGTGCAGTGATGGGGAGCGTGCCCGGGCTGAGCTCAGTGACAAGGTCCACAAGCTACAG
Seq A exon
GTGAGGGCAGGCAGCACGACCAAGCTGCTGCTCTCTAGCTGCCTGAAAGCTGGCTCCTCGGGATGCATCCTTGTATTCCCGTGTCAATTCTTCTTGTCAAAACCTCCTGCATATACTTAATGAACATGTATTGAGCACCTACTGTATACCAGAGACTGTGCTTCATCCTTAGACAAAAGATCAAGGAACATTACAGATTATCTCTGTAATCAGTGGGAGAGCTGGTCACAAACACAAAGAGAAAATCTAGGAGACACACATAATATTTAAGGGCAAATATAGGACAGCCTGGAGTGCAGGGGTGGAGTGGGGTCAGGAAAGGCTGCCCTTAGGAGGTCTAACACTCAGATGCATATGTATGCAGAAGGTATAGCATATGCAAAGGCCTGTGGTAAATGGGAAGAAGTCAAGGTCCAGCAGTGCTGACAAGTTGAGAGAAGAAATGGTATGAGAACAGGACCAGAGAGGAGAGCTGTGGCCAGACTACAAAGGGTCTGATGCCCCAGTCAGACATATGTGGTGTTTGCCCCAAGTAACAGAGGTAGCTAAAGCTGATGAATGACAGGGTCTGATGATCTTTGGTTTCTAGCTTCAAAGACACATTCGTTTGGCCTGAGTTCCCATTCCCAAAGAATGGTCCAAAATTCGATGAATATTTCCTGTGTTCACAAGCTGGAACAAGGGAGCAGGGAAACACCATGGTTGTCATTCCCTGCAGTTGAGAACAGATGGCCATGAAAGTTATGAGAAGGCAGTGAGCAGGACCTGTCTACAGTTGTGCTGTGTGTGTTTCTTCTCTAGTGGCTAGGAGTGCTTCCAGTACTGGAACTCTCAGATCTGCAGCTCATCTCTGCCAACCTTGGTAGGGATTTGGAACCCAATAAAGTGGATTCTAACTGAACTTAGTCTATACAGTGGAAGGATCTAGAACAGCCTAGTCTATACAGTGGAAGGGTCTAGAACAGAGTGTAGAGCCAGCATTCCCAGCTTGTAGCTGATGTGTTCCAGAGGATCCACAGTTGCCACTTGCCTCCTGTGTATTTTCTATCACTGGGTACCACAGGTCCTATCCCACGAAGGTGTGTGATGTATTCTGCATGAAAAGGGCTCCTCTTGCCGATCTGTGATTTGAGTTTCATCTCCCTGGTCTCTTGCTCTTCCCCATCCCCTCCAG
Seq C2 exon
AATGAAGTGGAGAGTGTCACTGGCATGCTCAATGAGGCAGAGGGCAAAGCCATCAAACTGGCCAAAGATGTGGCTTCCCTTGGATCCCAGCTTCAGGACACCCAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000018830-Myh11:NM_013607:28
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.870 A=NA C2=0.457
Domain overlap (PFAM):
C1:
PF0157614=Myosin_tail_1=FE(7.9=100),PF045827=Reo_sigmaC=FE(47.2=100)
A:
NA
C2:
PF0157614=Myosin_tail_1=FE(4.0=100),PF045827=Reo_sigmaC=FE(23.6=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGCAAACCTGGACAAAAGCAA
R:
TGTCCTGAAGCTGGGATCCAA
Band lengths:
302-1476
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: