DreINT0099816 @ danRer10
Intron Retention
Gene
ENSDARG00000100972 | myh11b
Description
myosin, heavy chain 11b, smooth muscle [Source:ZFIN;Acc:ZDB-GENE-101124-2]
Coordinates
chr3:36546735-36547937:-
Coord C1 exon
chr3:36547833-36547937
Coord A exon
chr3:36546888-36547832
Coord C2 exon
chr3:36546735-36546887
Length
945 bp
Sequences
Splice sites
5' ss Seq
CAGGTACTA
5' ss Score
7.64
3' ss Seq
TTTGTCTGTCCTCCTGTCAGGAG
3' ss Score
9.36
Exon sequences
Seq C1 exon
ACAGAGTATAACAATGTGAACAGCATCCTCAATGAGGCAGAGAGCAAGAACATCAAGCTGAGTAAAGACGTTGTCAGCCTTAACTCACAGCTCCAAGATGCACAG
Seq A exon
GTACTATATAACCACTAGCAAATGGTTTTTAGATTTTCATTCTTAGAGCATGACAATCCGCAAAGATCAGCAAATTCTGAAATACTTTGACCAACTCTGTATGGCATTGACAATCATCTTAAGTTTAAAGTCCCTTCAAGAGTTCACACTTAGCTGATAATTGATTATAAAGTGTGTTAGGCGTGCTGTCCTGGGAGAGAGCGCTGAGCTCATAAGATCCTCCAGCCCAGGACTCACTCCCCTTCCAGGGAGTTTGAGCTCAGGTAGACCTCGCGAACTCCCCTGCTGTTTATGGCTACAGAGAAATTCATAATTGCTATGTACTGCCGTCTAAGAGCTCAATGTGTGGTGCCAATTTGGTTTAGTCAATTCACTAATGCCGCATGCCTTTGGACGGTTGGAGTAAAACGGGGGAAGCCGGGGGAAACCTACACGAGCACAGGGAGAACATGTAAACTCCACACAGAAATGTCAACTGGCCTGAAAAGGACTTGAACCAGTGACAATCTTGCTTTGAGGCAACATTGCTAACCACTGGATCACCAGGAGTAGGGTGTAGGAGTAAGGGTGGAAGGGGGGATTCTTCAAGGTGAAGATCATTTATAAGCATGTGATTCTCTCGAAAATGGTTTATGAATAAACCTAAATTCCTTTTTCTAACTCATTCTGATGCTCAGTTTAACTTTTAGCAAATCTTCTTCACCCTATAAACATATACAATCGTAATAATTTGAATTTTGGTTTAGCAAGAAGTAGTTTATAATGTGCCTGGCGAGTGAAGCTACCTTTATATTTTAAAATAAGAAACTTTCAAAGCAGGATCGTCATAACGTGGAGTCATGCTTTTTGAAAAACTCTCATCTAAAATGAAGTGAACACCATAAGGTTATAGTGCATATTTTAAGATGCACCTGATTGATGATGATTTGTCTGTCCTCCTGTCAG
Seq C2 exon
GAGCTACTTGCTGAGGAGACACGTCAAAAACTTAACTTTTCCACTAGACTGCGTCAAATGGAGGATGAACGAAATGGTCTTCTGGAACAAATAGATGAAGAGACAGAAGCCAGAAGGAATGTGGAGAGACATGTCTCTAGTCTTAACACACAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000100972:ENSDART00000166896:27
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.457 A=NA C2=0.902
Domain overlap (PFAM):
C1:
PF0157614=Myosin_tail_1=FE(4.0=100)
A:
NA
C2:
PF0157614=Myosin_tail_1=FE(5.9=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACAGAGTATAACAATGTGAACAGCA
R:
TGTGTGTTAAGACTAGAGACATGTCT
Band lengths:
257-1202
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]