DreINT0099822 @ danRer10
Intron Retention
Gene
ENSDARG00000100972 | myh11b
Description
myosin, heavy chain 11b, smooth muscle [Source:ZFIN;Acc:ZDB-GENE-101124-2]
Coordinates
chr3:36545279-36545645:-
Coord C1 exon
chr3:36545484-36545645
Coord A exon
chr3:36545408-36545483
Coord C2 exon
chr3:36545279-36545407
Length
76 bp
Sequences
Splice sites
5' ss Seq
CAGGTCAGA
5' ss Score
7.41
3' ss Seq
CTTTATCTTCCCCATGACAGACT
3' ss Score
7.31
Exon sequences
Seq C1 exon
GTCCGTGAGCTTGAGGCTGAGCTAGAAGATGAGCAGAAGATGAGAACGTCCTTGGCTGCTGCCAAAAAGAAACTGGAAGGGGATTTGCAGGACCTTGAAGACCAGGTTGATGTAAACAGCAGAGCGAGGGATGAGGCCGTTAAACAACTCCGAAAGATTCAG
Seq A exon
GTCAGAAAGATAACTCTTCAAATTCTTTCAGAATTTCCCAAGCCATTCCCACCAGCCTTTATCTTCCCCATGACAG
Seq C2 exon
ACTCAGATGAAAGACTACCAGAGGGAGCTGGAAGATGCTCGAGCATCCCACAAGGAAGTTCTTTCCGATGCTAGAGAATCTGAGCGAAAGGCCAGAGCTATGGAGGCCGAAATCTTACACTTGCATGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000100972:ENSDART00000166896:32
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.981 A=NA C2=1.000
Domain overlap (PFAM):
C1:
PF0157614=Myosin_tail_1=FE(6.2=100)
A:
NA
C2:
PF0157614=Myosin_tail_1=FE(4.9=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GATGAGAACGTCCTTGGCTGC
R:
CTTCCTTGTGGGATGCTCGAG
Band lengths:
182-258
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]