DreINT0099897 @ danRer10
Intron Retention
Gene
ENSDARG00000076075 | myh7ba
Description
myosin, heavy chain 7B, cardiac muscle, beta a [Source:ZFIN;Acc:ZDB-GENE-100914-2]
Coordinates
chr11:24951467-24951885:-
Coord C1 exon
chr11:24951770-24951885
Coord A exon
chr11:24951660-24951769
Coord C2 exon
chr11:24951467-24951659
Length
110 bp
Sequences
Splice sites
5' ss Seq
CCGGTGAGT
5' ss Score
10.9
3' ss Seq
ACCTTTATTAACTTCTCTAGCCG
3' ss Score
6.35
Exon sequences
Seq C1 exon
GGCACTCTGGAGCATGAAGAGAGCAAGACTCTACGTATACAGCTGGAGCTCAACCAGATCAAAGCAGACACTGACCGGAAGCTGGCAGAGAAAGATGAGGAAATTGACAATCTCCG
Seq A exon
GTGAGTTTAGTTTAATGAGACAAGTCTGAGAACTGATGAAGATCTTCAGCGAATCTGCTATACAAACAAAAAGGCTCCCTAATAAATTTAACCTTTATTAACTTCTCTAG
Seq C2 exon
CCGTAACCATCAGCGTGCCCTAGAGTCCATGCAAGCCACGCTGGATGCAGAAGCAAAATCTCGTAGTGAAGCCATTAGGGTGAAAAAGAAGATGGAGAATGACCTGAATGAAATGGAGGTTCAGTTGAATCATGCAAATTGGTTGGCCACTGAGTCCCAGAAAATGGTCCGAAACCTACAGACACAGATTAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000076075:ENSDART00000097316:34
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.744 A=NA C2=0.892
Domain overlap (PFAM):
C1:
PF0157614=Myosin_tail_1=FE(4.4=100)
A:
NA
C2:
PF0157614=Myosin_tail_1=FE(7.5=100),PF0003816=Filament=PU(3.6=15.4)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CAAAGCAGACACTGACCGGAA
R:
TCTGTGTCTGTAGGTTTCGGAC
Band lengths:
245-355
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]