DreINT0100006 @ danRer10
Intron Retention
Gene
ENSDARG00000001014 | myh9b
Description
myosin, heavy chain 9b, non-muscle [Source:ZFIN;Acc:ZDB-GENE-030131-998]
Coordinates
chr3:5233168-5233704:-
Coord C1 exon
chr3:5233581-5233704
Coord A exon
chr3:5233340-5233580
Coord C2 exon
chr3:5233168-5233339
Length
241 bp
Sequences
Splice sites
5' ss Seq
AGGGTGAGA
5' ss Score
6.65
3' ss Seq
TGTGTCGTATCCTCCTCCAGACC
3' ss Score
10.37
Exon sequences
Seq C1 exon
GAGAAGAAGCTCATGGAGGAGCGGATCGCAGAGTTCACCACAAACCTGGCGGAGGAGGAGGAGAAGTCCAAGAGTCTGCAGAAACTGAAGAACAAACACGAGGCCATGATCACCGACCTGGAGG
Seq A exon
GTGAGAGCTCCACTTTCACTTTCAACATTACATTCATTAGCAGATGCTTACACATTTTAAGGTTTTCAGTTCATTGCAAGTCTCTCTACATAGAGTATTTCCTTAAATTCATATCCACTGGGCTCTATTAGACGATTTTCTGCTTATATTAAGATGTGATGTTTGAATATTGATAGTTTTCTGCAATATGTAATTAATGTGTAAGGTGACTGTATTTTAAATGTGTCGTATCCTCCTCCAG
Seq C2 exon
ACCGTCTGCGGCGCGAGGAGAAGCAGCGCCAGGAGCTGGAGAAGAACCGCAGAAAGCTGGAGGGAGACTCGACAGAGCTGCACGACCAGATCGCAGAGCTCCAGGCGCAGATCGCAGAACTCAAAGCGCAGCTCGCCAAGAAGGAGGAGGAGCTGCAGGAGGCACTCGCCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000001014:ENSDART00000137105:23
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.929 A=NA C2=0.724
Domain overlap (PFAM):
C1:
PF0157614=Myosin_tail_1=FE(17.4=100)
A:
NA
C2:
PF0157614=Myosin_tail_1=PD(16.5=67.2),PF093045=Cortex-I_coil=PU(48.9=75.9),PF0157614=Myosin_tail_1=PU(4.0=43.1)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GAGAAGAAGCTCATGGAGGAG
R:
CTCCTGCAGCTCCTCCTCC
Band lengths:
285-526
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]