DreINT0100020 @ danRer10
Intron Retention
Gene
ENSDARG00000001014 | myh9b
Description
myosin, heavy chain 9b, non-muscle [Source:ZFIN;Acc:ZDB-GENE-030131-998]
Coordinates
chr3:5261434-5261678:-
Coord C1 exon
chr3:5261581-5261678
Coord A exon
chr3:5261498-5261580
Coord C2 exon
chr3:5261434-5261497
Length
83 bp
Sequences
Splice sites
5' ss Seq
TTTGTAAGT
5' ss Score
7.4
3' ss Seq
TCATCTGCTCTGTGTTTCAGGGC
3' ss Score
11.84
Exon sequences
Seq C1 exon
CGCAGGGCGAGCTGGAGAAGCAGCTGCTGCAGGCAAACCCGATCCTGGAAGCCTTCGGAAACGCCAAGACCGTCAAAAACGACAACTCGTCTCGATTT
Seq A exon
GTAAGTCTCTCTTGCTTTATATCTTATTTCAGTTCCTGTGTAAATACATGTTCCATAGATCTTTCATCTGCTCTGTGTTTCAG
Seq C2 exon
GGCAAATTCATCAGAATCAACTTTGACGTCAACGGCTACATCGTTGGAGCCAATATTGAAACCT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000001014:ENSDART00000137105:6
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.037 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0006316=Myosin_head=FE(4.4=100)
A:
NA
C2:
PF0006316=Myosin_head=FE(3.1=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCAGGGCGAGCTGGAGAA
R:
AGGTTTCAATATTGGCTCCAACGA
Band lengths:
161-244
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]