DreINT0100029 @ danRer10
Intron Retention
Gene
ENSDARG00000095930 | myha
Description
myosin, heavy chain a [Source:ZFIN;Acc:ZDB-GENE-060531-50]
Coordinates
chr5:31655264-31655894:+
Coord C1 exon
chr5:31655264-31655572
Coord A exon
chr5:31655573-31655690
Coord C2 exon
chr5:31655691-31655894
Length
118 bp
Sequences
Splice sites
5' ss Seq
AAGGTATGA
5' ss Score
9.11
3' ss Seq
AAAGATGTTGTTGTGTTCAGGAT
3' ss Score
6.66
Exon sequences
Seq C1 exon
GGCACCCTGGAGCATGAGGAGTCCAAGATTCTTCGTGTCCAGCTTGAGCTAAACCAGGTCAAGGGTGAGATTGACAGGAAGCTTGCAGAGAAGGATGAGGAGATAGAGCAGATCAAGAGGAACAGTCAGAGAGTTACTGAAGCCATGCAGAGCACTCTGGACTCTGAAGTCAGAAGCAGGAATGATGCTCTCAGAATCAAGAAGAAGATGGAGGGAGACCTTAATGAAATGGAGATTCAGCTGAGCCATGCCAACCGCCAGGCTGCTGAGGCCCAGAAACAGCTCAGGAATGTTCAGTCACAACTCAAG
Seq A exon
GTATGACTTTGTTGTGTCATTGGGGCACATATATGTGTTTGTTTAAGCTAAACTATGTTAAGTAAAAGTCAACTGAAACCAGATAAAAATTTGCTTACAAAGATGTTGTTGTGTTCAG
Seq C2 exon
GATGCCCAGCTGCACCTTGATGATGCTGTGAGAGCACAGGAAGACATGAAGGAGCAGGTGGCCATGGTGGAGCGCAGAAACACTCTGATGCAGTCTGAGATCGAAGAGCTGAGAGCTGCTCTGGAGCAGACAGAGAGAGGACGCAAAGTGGCTGAACAAGAGCTGGTGGATGCCAGTGAGCGTGTTGGACTTTTGCACTCTCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000095930:ENSDART00000139917:33
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.806 A=NA C2=0.838
Domain overlap (PFAM):
C1:
PF135141=AAA_27=PD(4.3=31.1),PF0157614=Myosin_tail_1=FE(11.9=100),PF0003816=Filament=PD(11.1=33.0),PF104734=CENP-F_leu_zip=PU(23.6=32.0)
A:
NA
C2:
PF0157614=Myosin_tail_1=FE(7.8=100),PF104734=CENP-F_leu_zip=FE(47.9=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GATTCAGCTGAGCCATGCCAA
R:
GCATCCACCAGCTCTTGTTCA
Band lengths:
249-367
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]