DreINT0100034 @ danRer10
Intron Retention
Gene
ENSDARG00000001993 | myhb
Description
myosin, heavy chain b [Source:ZFIN;Acc:ZDB-GENE-081124-1]
Coordinates
chr6:40756241-40756549:-
Coord C1 exon
chr6:40756451-40756549
Coord A exon
chr6:40756345-40756450
Coord C2 exon
chr6:40756241-40756344
Length
106 bp
Sequences
Splice sites
5' ss Seq
TGGGTAAGA
5' ss Score
8.91
3' ss Seq
TTGCTGTATTCCTCTCGTAGAGG
3' ss Score
7.62
Exon sequences
Seq C1 exon
ATCTGCTGGAAAAGTCCAGGGTAACTTTCCAGTTGTCTGCTGAGAGGAGCTACCACATCTTCTACCAGCTGTGCACAGGCCATAAGCCAGAACTGCTGG
Seq A exon
GTAAGAAAATGTACATCACTATATTAGAATGAGACAAGAGCATAGACAAGACTTATATTTGTACAGCAGATCAAAGTCTTCATTTCTTGCTGTATTCCTCTCGTAG
Seq C2 exon
AGGCTCTTCTGATCACCACTAACCCATACGACTATCCCATGATCAGTCAGGGTGAGATCACAGTCAAGAGCATCAACGATGTGGAAGAGTTCATTGCAACTGAT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000001993:ENSDART00000156295:10
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0006316=Myosin_head=FE(4.8=100)
A:
NA
C2:
PF0006316=Myosin_head=FE(5.0=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGAAAAGTCCAGGGTAACTTTCCA
R:
TCAGTTGCAATGAACTCTTCCACA
Band lengths:
195-301
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]