DreINT0100049 @ danRer10
Intron Retention
Gene
ENSDARG00000001993 | myhb
Description
myosin, heavy chain b [Source:ZFIN;Acc:ZDB-GENE-081124-1]
Coordinates
chr6:40752432-40752881:-
Coord C1 exon
chr6:40752705-40752881
Coord A exon
chr6:40752578-40752704
Coord C2 exon
chr6:40752432-40752577
Length
127 bp
Sequences
Splice sites
5' ss Seq
GATGTGAGT
5' ss Score
7.77
3' ss Seq
CTTTCCACATCTGCAAATAGCTG
3' ss Score
4.78
Exon sequences
Seq C1 exon
GTTAAAAACCTTACCGAAGAAATGACATCTCAAGATGAGGTTATTGTAAAGCTGACTAAAGAAAAGAAAGCCCTCCAAGAGGCACATCAGCAAACTTTGGATGATTTACAGGCAGAGGAAGACAAAGTCAATACTCTGACGAAAGCCAAAGCAAAGCTTGAGCAGCAAGTTGATGAT
Seq A exon
GTGAGTTTGAAATTGAACAGATTTGTGCAAATTCTCCTGTTTATTTACTTTTACAAGAAGCAGAACTCAACCACAGTAGTGATGTTTTCAATATCATTTTCCTTAATCTTTCCACATCTGCAAATAG
Seq C2 exon
CTGGAGGGATCTCTAGAGCAGGAAAAGAAACTACGTATGGACCTCGAGAGAGCCAAAAGAAAGCTTGAAGGTGATTTAAAACTTGCCCAGGAATCTTTAATGGACTTGGAAAATGACAAGCAGCAACTAGAAGAGAAATTGAAAAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000001993:ENSDART00000156295:24
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.576 A=NA C2=0.469
Domain overlap (PFAM):
C1:
PF0157614=Myosin_tail_1=FE(43.0=100),PF0003816=Filament=PU(6.6=32.2)
A:
NA
C2:
PF0157614=Myosin_tail_1=PD(25.9=71.4),PF0003816=Filament=FE(16.8=100),PF0157614=Myosin_tail_1=PU(1.6=28.6)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTCCAAGAGGCACATCAGCAA
R:
TTCTCTTCTAGTTGCTGCTTGTCA
Band lengths:
242-369
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]