DreINT0100064 @ danRer10
Intron Retention
Gene
ENSDARG00000001993 | myhb
Description
myosin, heavy chain b [Source:ZFIN;Acc:ZDB-GENE-081124-1]
Coordinates
chr6:40748588-40748880:-
Coord C1 exon
chr6:40748776-40748880
Coord A exon
chr6:40748684-40748775
Coord C2 exon
chr6:40748588-40748683
Length
92 bp
Sequences
Splice sites
5' ss Seq
CAGGTAATA
5' ss Score
8.55
3' ss Seq
TTTAAAATGTTTTCTGACAGACT
3' ss Score
7.46
Exon sequences
Seq C1 exon
GTTCGTGAACTGGAGAGTGAAGTCGAAGCTGAACAGAGACGTGGTGCTGATGCTGTGAAAGGAGTCCGCAAATATGAAAGAAGAGTGAAGGAACTGACTTATCAG
Seq A exon
GTAATAATAATCACACAAATAAACGTATATAGGTGTTATAAGTCAAATACAGTCAAATCAAAATGGTTATCTTTTAAAATGTTTTCTGACAG
Seq C2 exon
ACTGAGGAAGATAAGAAGAATATTATTAGACTGCAAGACCTTGTGGACAAACTTCAACTGAAAGTCAAGGCCTACAAGCGACAGAGTGAAGATGCT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000001993:ENSDART00000156295:38
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.514 A=NA C2=0.219
Domain overlap (PFAM):
C1:
PF0157614=Myosin_tail_1=FE(3.9=100)
A:
NA
C2:
PF0157614=Myosin_tail_1=FE(3.6=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CGAAGCTGAACAGAGACGTGG
R:
CTCTGTCGCTTGTAGGCCTTG
Band lengths:
168-260
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]