DreINT0100796 @ danRer10
Intron Retention
Gene
ENSDARG00000010186 | myo3a
Description
myosin IIIA [Source:ZFIN;Acc:ZDB-GENE-041026-4]
Coordinates
chr24:6190228-6190614:-
Coord C1 exon
chr24:6190459-6190614
Coord A exon
chr24:6190264-6190458
Coord C2 exon
chr24:6190228-6190263
Length
195 bp
Sequences
Splice sites
5' ss Seq
AAGGTAGAT
5' ss Score
7.24
3' ss Seq
CTTTATTTCTATTGTTTAAGCCT
3' ss Score
5.02
Exon sequences
Seq C1 exon
GTGTCTGATAAAGGATTTTGAGCTCAGGCCCAATGTGCTTGACCTTCTTCAGCATGTCTTCATCAAACAGATTGTGGGCCGAGAGAAGACGCTGCAGAAGCAGCTGATGGAGCTAATCGACCTCAACCAGCAAATGGGCATCATTGAGAAAACAAG
Seq A exon
GTAGATGCAAATATCAGCCCTAATGTTTTCTGAATCATGCAGTGTGTCTGACTAGACAAAAACAAAACAGCATGACTAGTTTGTGTCGGCACTTTATTTCTACCACGTGCAATTTACCAGATAGAGAGTAGCAAGACCCCCATCATTACATTATAGATGTCTCTTGAAATTGTAACTTTATTTCTATTGTTTAAG
Seq C2 exon
CCTTCTCGGAAAATCTGACAGGGACAGTGACTCCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000010186:ENSDART00000136041:9
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.019 A=NA C2=0.821
Domain overlap (PFAM):
C1:
PF0006920=Pkinase=PD(7.9=39.6)
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GATAAAGGATTTTGAGCTCAGGCC
R:
CTGGAGTCACTGTCCCTGTCA
Band lengths:
186-381
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]