DreINT0100827 @ danRer10
Intron Retention
Gene
ENSDARG00000061635 | myo5aa
Description
myosin VAa [Source:ZFIN;Acc:ZDB-GENE-041027-2]
Coordinates
chr18:39010663-39011127:+
Coord C1 exon
chr18:39010663-39010771
Coord A exon
chr18:39010772-39010915
Coord C2 exon
chr18:39010916-39011127
Length
144 bp
Sequences
Splice sites
5' ss Seq
CAGGTACAA
5' ss Score
7.09
3' ss Seq
TTTTTCTTTCCTCTTGATAGGAT
3' ss Score
11.03
Exon sequences
Seq C1 exon
GTGGACGTATCAAGAGTTCTTTAGCAGGTATCGTGTACTGATGAAACAAAAAGATGTTCTGACGGACAAGAAGATGACCTGTAAGAATGTTCTGGAGAAACTGGTGCAG
Seq A exon
GTACAAGTTGAAGACTGATACTAGTCTATATGGGTGAACTCAATACAAAGAGCATAATGCTGTAAAGTAGCTTTAAAACACTCAGTATGATTGCAACATTAACAGCAGTCTTATTTGTGTCTTTTTTTTCTTTCCTCTTGATAG
Seq C2 exon
GATCCAGATAAGTACCAGTTTGGAAAGACCAAGATTTTTTTCCGCGCAGGTCAGGTTGCATACCTGGAGAAACTGCGTGCTGATAAGCTGCGCGCTGCCTGTATCCGCATCCAGAAGACCATCCGCTGCTGGCTGGCACGCAAGAAGTACCTGCGTATGAAGCATGCTGCCACGACTATCCAGAGATTCGTCCGTGGATACCAGGCCCGCTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000061635:ENSDART00000133618:18
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0006316=Myosin_head=FE(5.3=100)
A:
NA
C2:
PF0006316=Myosin_head=PD(2.1=19.7),PF0061222=IQ=WD(100=29.6),PF0061222=IQ=PU(81.0=23.9)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGACGGACAAGAAGATGACCTG
R:
TCCACGGACGAATCTCTGGAT
Band lengths:
248-392
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]