DreINT0100844 @ danRer10
Intron Retention
Gene
ENSDARG00000061635 | myo5aa
Description
myosin VAa [Source:ZFIN;Acc:ZDB-GENE-041027-2]
Coordinates
chr18:39054100-39054486:+
Coord C1 exon
chr18:39054100-39054254
Coord A exon
chr18:39054255-39054396
Coord C2 exon
chr18:39054397-39054486
Length
142 bp
Sequences
Splice sites
5' ss Seq
AAGGTCTCG
5' ss Score
0.08
3' ss Seq
CCTTTCCCATAACTCCACAGAAA
3' ss Score
8.91
Exon sequences
Seq C1 exon
AGCTGAAGCCTCGTGGTGTTGCAGTGAATCTGATACCAGGTCTGCCCGCATACATCCTCTTCATGTGTCTGCGCCATGCTGACTACATCAACGATGACCAGAAAGTGCGATCTCTACTCACCTCCGTCATCAACAGCATCAAGAAGATCCTCAAG
Seq A exon
GTCTCGAATCACTCACGCAATTCATGTTTATAATGTTGCAACTTACAAAATTTACAAACTGTAAATTTGTATTATAAAAGCATAATCACTGTTAAGAGTAAATAATTAAGAATTGTTGTTTCCCTTTCCCATAACTCCACAG
Seq C2 exon
AAACGAGGTGATGACTTTGAGACTGTTTCCTTCTGGTTGGCCAACACATGTCGTTTTCTGCACTGCCTGAAACAGTACAGTGGTGATGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000061635:ENSDART00000133618:33
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF058146=DUF843=PU(83.3=86.5)
A:
NA
C2:
PF058146=DUF843=PD(13.0=23.3)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGCTGAAGCCTCGTGGTGTT
R:
ATCACCACTGTACTGTTTCAGGC
Band lengths:
242-384
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]