DreINT0100872 @ danRer10
Intron Retention
Gene
ENSDARG00000074622 | myo5ab
Description
myosin VAb [Source:ZFIN;Acc:ZDB-GENE-050411-72]
Coordinates
chr25:7440301-7440655:-
Coord C1 exon
chr25:7440471-7440655
Coord A exon
chr25:7440372-7440470
Coord C2 exon
chr25:7440301-7440371
Length
99 bp
Sequences
Splice sites
5' ss Seq
TGTGTGTGT
5' ss Score
-1.13
3' ss Seq
TGTTTTTCAGATTGTAAAAATCC
3' ss Score
-11.53
Exon sequences
Seq C1 exon
GTATAATGTTTGTCAGCTGGAGGAGTGGCTGCTGGATAAAGATCTTCAGGGCAGTGGAGCTCGAGAGTCTCTGGAGCCTCTTATTCAGGCGGCGCAGCTCCTGCAGATCAAGAAGAAGAGCCAGGACGACGCTGACGCCATCTGTACTATGTGCACAGCGCTCACCACTCAACAGGTCTGTGTGT
Seq A exon
GTGTGTCAGAGTTACTCAGATGAATGCTTGAGTTTATTTATGATGTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTGTGTTTTTCAGATTGTAAAAA
Seq C2 exon
TCCTCAGCTTGTACACACCGGTGAATGAGTTTGAGGAGAGAGTGTCTATATCATTCATAAAGACCATACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000074622:ENSDART00000089034:42
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0184314=DIL=FE(58.5=100)
A:
NA
C2:
PF0184314=DIL=PD(9.4=41.7)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Rat
(rn6)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATCTTCAGGGCAGTGGAGCTC
R:
TTCACCGGTGTGTACAAGCTG
Band lengths:
169-268
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]