GgaINT1018725 @ galGal4
Intron Retention
Gene
ENSGALG00000004624 | MYO5A
Description
unconventional myosin-Va [Source:RefSeq peptide;Acc:NP_990631]
Coordinates
chr10:8328295-8329833:+
Coord C1 exon
chr10:8328295-8328469
Coord A exon
chr10:8328470-8329752
Coord C2 exon
chr10:8329753-8329833
Length
1283 bp
Sequences
Splice sites
5' ss Seq
CAGGTAATA
5' ss Score
8.55
3' ss Seq
ATGGTTGTTGTTCTTCACAGATT
3' ss Score
9.14
Exon sequences
Seq C1 exon
GTACAATGTGAGTCAACTTGAAGAATGGCTACGTGATAAAAATCTAATGAACAGTGGGGCCAAAGAAACACTGGAGCCCCTCATACAGGCTGCACAGTTGTTGCAAGTGAAAAAGAAAACAGATGAAGATGCAGAAGCCATTTGTTCAATGTGCAATGCACTGACTACTGCCCAG
Seq A exon
GTAATAACCTCTCATTCTCTTTTTGCTGTTTCTTTTGAGCAGTTCTCTGTTCTGCTGTGTTTGCTAAGCCTGCCTGCACCGCTGTTGCAGACAGGTCATACATTGCTAACATTGCTTTGAGTCTGTATAAGTAAACAGATTAAATTGGAGCGGTGAAAAAAAGGACTAGGTCTAGTGCTCAAGATAAGTTTTGACCAATTGAGAGGAAACTCAGCACACTGTGCAGTGTGTAGCACTATAATTAGTCTCAGGATGCAAAAATCCTCAGAGAAAAGCCTTCACTGGCATCTCTGTTCCCCAGGTAACTCTGCCTAGGATTCTAGGAAATTATCTTTCTGCCATCTGAGTTGGCATCCATTTAAGATAGTGGACAATTCAGGCAGAGCTGTTGTCTCAGGGTTTGCAAACAGGAGTATTAAGTTCCTGTACCCATTAGCCTCAAAAAGGCTGAGCCAAGTGTATCTCAGTTGGCAGCACAGCTGAAGGCTTCTCCATAGGAAAGCTTAGGTTTTGCAAAGACAAGCTGCACTTATCTTAACTGTGCAACATATGCTTCGAATTTTTATGGTAATAACAGGATCCAAGCATCTTTGGTTATTACTGAACATTCTTATCAGGAAAAGAAACAAATTTCCGTTCCTTTCATAGTGATGTCAGCAGGAGCAGTGACATTGATACAGCTGTTTGGATTTAGTAATGCACTGATTTAGTTCTGAACTGAAAGAAGGTATAATTCTATGGGCTTTGAGTGTTTTCTGTTGAGAAGATTTGTTTTTTAAACAAAAAAGTAAAAGATTGACTTATTTATGTAAGTTTTATATGTTTAATTCATTACTGTTCAAAGGCTCTGTGTTGCAGTTTAGACTTCTGGTGACAATAGCCCTGCTTTTAGAGAAATAGCTCACTCCCTTGAAAAACACTGAGGTCATAAGATGCTCTTGAGTGAAAGCTGCAGGAGGCCATAGGGAGTCAAAGCTAGGAAGAGAAGTGCACTTTGTTTCCAGAGCATGTAGGAATGCAGTGAGCTGTTTATCATCTGACTTTGCCACAGTTTGGTGTTTTCCCCATGCATGGAAGAATTGGAGTTTCTACCTCCAAAGAACACGTGTTCTAAGGGGGCAGGTTTAAAAATACCTTTGAAGAGATAATGACATTTTGTATGAATCAGTAAAACAAACAAAAAGAAATACTTTAGATTTTAACAATAGCTCATGTAAAAAATGTATCAGTAACTGTAGACTTATTAGTAGAGTGAAACTAAGGATGGTTGTTGTTCTTCACAG
Seq C2 exon
ATTGTGAAAGTACTGAATTTGTATACTCCAGTTAATGAATTTGAAGAGAGAGTCTTGGTATCGTTTATACGTACAATACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000004624:ENSGALT00000039066:42
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0184314=DIL=FE(54.7=100)
A:
NA
C2:
PF0184314=DIL=PD(12.3=48.1)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CAATGTGAGTCAACTTGAAGAATGGC
R:
AAACGATACCAAGACTCTCTCTTCA
Band lengths:
238-1521
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]