DreINT0100882 @ danRer10
Intron Retention
Gene
ENSDARG00000062003 | myo5b
Description
myosin VB [Source:ZFIN;Acc:ZDB-GENE-031219-7]
Coordinates
chr21:22448638-22448991:-
Coord C1 exon
chr21:22448905-22448991
Coord A exon
chr21:22448750-22448904
Coord C2 exon
chr21:22448638-22448749
Length
155 bp
Sequences
Splice sites
5' ss Seq
ACGGTAATC
5' ss Score
4.8
3' ss Seq
CATCTGATGTTTTTTCTTAGATG
3' ss Score
8.44
Exon sequences
Seq C1 exon
GTTTGATTCTAGAAGAGCTGTTCAGCAACTAAGAGCATGTGGTGTTCTGGAGACCATTCGCATTAGCGCCGCCGGCTATCCATCACG
Seq A exon
GTAATCAATCTACAGCTCTTTTCTTTCTTGTTTGATTTATCTGACTTCAGTTTATAAACCTCAGGGTCTCCCCAGTAGAATTCCATAATATTTCTGTATTGACTTCAATAGTCATTCAAACTGATTTCCTCTTCACATCTGATGTTTTTTCTTAG
Seq C2 exon
ATGGACATATCCAGATTTCTTCAGCAGATATCGAGTGCTCATGACAAAGAAGGATCTGTCAGTCGGAGACAAGAAGCAGGTCTGTAAGAACCTGCTGGAGATCTTGATCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000062003:ENSDART00000166835:17
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0006316=Myosin_head=FE(32.6=100)
A:
NA
C2:
PF0006316=Myosin_head=FE(41.6=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGATTCTAGAAGAGCTGTTCAGCA
R:
TGATCAAGATCTCCAGCAGGT
Band lengths:
194-349
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]