DreINT0101024 @ danRer10
Intron Retention
Gene
ENSDARG00000099871 | myo7aa
Description
myosin VIIAa [Source:ZFIN;Acc:ZDB-GENE-020709-1]
Coordinates
chr18:3132127-3134002:+
Coord C1 exon
chr18:3132127-3132264
Coord A exon
chr18:3132265-3133843
Coord C2 exon
chr18:3133844-3134002
Length
1579 bp
Sequences
Splice sites
5' ss Seq
ATGGTGAGT
5' ss Score
10.13
3' ss Seq
GTCCGTCTCTTGGACTCCAGCTC
3' ss Score
5.35
Exon sequences
Seq C1 exon
GGAGCAGAGACCCGCAAGCGTTCTCCGACTCTGAGCAGTCAGTTCAAGCGTTCTCTGGAGCTGTTAATGCGCACACTGAGCGTCTGCCAGCCGTTCTTCGTCCGCTGCATCAAACCCAATGAATACAAGAAACCCATG
Seq A exon
GTGAGTTAAACCTGCATTATACAGCGATAAACTCTCTCATAACACTCTACAGTAAAGTCATGTTTCCTTTTAGCTCACATTTTACAATAGGGTTTCATTAGTTATTGTATTTATTAACATGAACTAATAATGAACATTACCTGTAGTGCATTTAATAATCACAGTTCAACATTTACTAATGCATTATTAACATCTAAATAATGTCTGTTAACATTACTTAATGCACTAGGAGTTAACAAGAACTAACAATAAACAACTTTTATTAACTGGTAACACTTTACAATAAGTTTATATTAGTTTATGTTAGTTAATGCATTAATAAACATGAAAAAACAATGATCAATATATTTATTACTGTATTTGTTCAAGTTAGTTAATGAAAACAAGCATGAATTTCAATGTTAATATGCATTAGTAAATGTTAAACTGTGATTAATAAATGCTGCATGAGTATTTATAATTATTAGTTGATGTTAGTAAATACATGAACTACCATTAAGTAATGAAACCTTATTGTAAAGTGTGACCCATTAACTAACATCACCAAAGATGAATTAATACTGTATTAAATGTATTGTTCATGTTAGCAAATTCACTGTATGTGCTATATTATTACAGCATTTCTATTTTTAAAGGTGCTGTATGTAAGTTTTTGACTCTTCTAAAGCATTAATCACCATAAAATGTTTGCAGATATTTAGGAAACATGCTCGGTGAACATTCTTGTTTATCTGAAAAACAATGCTAGTCAAATATTTTGCTTTGAAAATGTGTTTTCCGCGCCCAAACGGGTGTCTTTGTTTTGGTCATTTTAACCTGCCCACTGTCAGTTTAGCCAATTACATTTCAGCAGCCCGGGTTGCCTTGGTGGAAAATCGCAAATTCCATTTATTCATTCAGAAAGGCTCTGAAAGTATGCGTCTGTGATTGAAATGCGACCTCTGGTGGACAGTAGCGGACTCCCAAACGAGACGCACATTCAGTTCCATATGAGGTGGTTATTAATTAGCAAATGATATTAATATTACGAGCATAAACATTAAGTGAGCAGGTTACACTGTAACCCTGTGTCCTAACAACATGCATGACAGAAACACGACAGAAATTTAAAAACAGCCATTTAGAGGCACAGAATATGCAATCACTCACGAAATTGTTAAAGTTTATAATCTCTTTAATACATATTAACCCTCTTTAACGGTATTAAATGTAGATGCTGAATCACACATATGTGTTTAGTTCTAAAGTTTCCAGATCTGAGGTGAACTATCTGCTGCTGCTTTCAGTAGTATGGTTAAAAAAAACTGCTTTTATTCTAGCCAAAATAAAAAACAATTAAGACTTTCTCCAAAAGTAAAAATATTATCGGAAATACTTTGAAAAATTCCTTGCTCCGTTAAACATCTTTTGGGAAATATTTAAAAAAGAAATTTACCAGAGGGTGAATAATTTTGACATCGAGTGTGCATGTTATATTTATATAAAACAATTTTTGGTAATGATATTGCCGTAACAGTGATACTACCGAAAATTAATTATGACTATGAGTCTAATAATCTGTCCGTCTCTTGGACTCCAG
Seq C2 exon
CTCTTTGATCGTGAGCTGTGTGTGCGGCAGCTCCGTTATTCTGGCATGATGGAGACCATCCGGATTCGCCGGGCCGGATACCCCATTCGATACACGTTTGTGGAGTTTGTGGACCGGTACCGTGTTTTAATGCCTGGAGTGAAACCTGCTTACAAACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000099871:ENSDART00000166980:15
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.011 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0006316=Myosin_head=FE(6.8=100)
A:
NA
C2:
PF0006316=Myosin_head=FE(7.8=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GAGCAGAGACCCGCAAGC
R:
TGTTTGTAAGCAGGTTTCACTCCA
Band lengths:
295-1874
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]