DreINT0101067 @ danRer10
Intron Retention
Gene
ENSDARG00000044632 | myo7ab
Description
myosin VIIAb [Source:ZFIN;Acc:ZDB-GENE-091110-4]
Coordinates
chr21:22240894-22242749:+
Coord C1 exon
chr21:22240894-22241029
Coord A exon
chr21:22241030-22242642
Coord C2 exon
chr21:22242643-22242749
Length
1613 bp
Sequences
Splice sites
5' ss Seq
AAGGTACAC
5' ss Score
7.56
3' ss Seq
CCTCTTGTTTCATTGTTCAGGTT
3' ss Score
9.61
Exon sequences
Seq C1 exon
GGAACAGATGCAACCATGCTGAACAAGCTAAACTCCCAGCACAAACTTAATACCAACTACATCCCGCCTAAGCACAGCCATGAGACTCAGTTCGGCATTCAGCACTTTGCTGGAGTGGTGCACTATGAAACTAAAG
Seq A exon
GTACACATTATTGAATTACTGAGCTGTTGGTAATCTAAGTCAAGTCATTAGCATTTCTATTGCACTTTATACAGTACAGATTGTGTCAAGCAGCTTTTCAGAAAGTCAATTATCAAGATTCTTTAACACCATACATATAGATTTAAGACCAGCATTTCAGAGTGAGATCATTTTATTGTAGATAATAGCAAATACTGTATGACTTACATATGTATAATTCTGAAAACAATAAATAAATGTATTTTAAAATATATTCGTTCAGGTTGTGGTAAGTTAGATTGGTTTTAAGTGTCATTTATAATCATTCATAATCAAATTATGTCATTTTAAATGTCACATTGTTGGAGACGTCTTTGTCGTGACAACTTGACATCGCAAGTCAAAATACCTTGTCATAAATCTGTCATTAACATGACACTCTGAGGCATTATAATTGTCTGATCACTTTTTTAAGTAAAACAAACCGAATTTGTCATCGAAATCTCAATAAAGTGTCAATACTCTAAAATAGTTGTATAATATTAATATTTAATAACATTTTAATAATAAATTCAAATTGTACCAGTGTAATTAAGGTCAAGAAAAGTGACGCAATTATAATGGCCTTCTGACAGTCAAGTTTTTTGCTAGATTTGTAACATATTTTGTTTTCTTGGTCATGTCAAATTGTCATAAAAAAGACAAAGACAGATTTTGATGTTTGTGTCAAGTTATCATAACAAATATATCTGTCATGTTTGCTTTTAAAACGATAATAACTTAGTGACAGTTGTCATAAGCATGCATGAAATCTAATTTAGTTTAGGGAAGTGGGTGGGTGGGATAACCAGTGCTTTTGAAAACACTATTGGTTGGGAGTAGGGAAGGTGAAGGGTGTGCCAGTCGATTGGTCAGTCATTTATTCAGTTAGTCAACTGTGGCCTCTGGTGGATTTACGTGACTACAGGCGCTAATGGCACTAGCGAGAGAAATTTGGGATCTCAAAAACATACACAACGGCTTCTGGTGGATTCGCCAAAACAAAAACTGCAAAACGTAGCTCCTGGGACCAGAAATGTATACAAGGGTATGTAATAAAAAAAACATACTTCCTGGGACTCTCTAGAATTGTACATAGCGCTATGTTTTCAAAATGAGCCTGGGTTGCCCTTCTAATGAAGTGTTACCTAACCTTTCATAGCGCAATTATTGAAACACTTAGAAAAACCCTGGAGTTTTACTTGGGGTTTATTACAAAACTTTTGAACTTTTTCGACTAAAAAATAATTTTACTGTAATCAATGTCAATGAAGTTACTATATATGATTCATCTCCAGATAAAGGGTTAATAGATGAAGAACAGGTTAATTAAAAGTGACAGTAAAGACATTTATAAAGTTACAAATCATTACTAGTTCAATTAAGGATTTGTTTCAGGGAGTGGTTTCAGGCAGTTTTTAACCACATTAAAGTTCCATACATTTCAGGGTTTTATTTAAAGGCCATGTCAGCCTACAACAAAGAAATAAAAGCCTGTGAAGACACTGAGCAATCATATTAAATATTACAGCAGTTACAAAAGACATTTTTTTCTATTTTCATGAAAATGTAAAACCTCTTGTTTCATTGTTCAG
Seq C2 exon
GTTTCCTGGAGAAGAACCGAGACAGTCTCCACAGTGACATCATCCAACTCGTCCATTCATCCAAAAACAAGTTCATCAAGCAGATCTTCCAAGCTGATGTTGCCATG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000044632:ENSDART00000148019:12
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.188 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0006316=Myosin_head=FE(6.9=100)
A:
NA
C2:
PF0006316=Myosin_head=FE(5.3=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGAACAAGCTAAACTCCCAGCA
R:
TGGCAACATCAGCTTGGAAGA
Band lengths:
222-1835
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]