DreINT0101119 @ danRer10
Intron Retention
Gene
ENSDARG00000077201 | myo7bb
Description
myosin VIIBb [Source:ZFIN;Acc:ZDB-GENE-070912-482]
Coordinates
chr2:23253867-23255739:-
Coord C1 exon
chr2:23255521-23255739
Coord A exon
chr2:23254068-23255520
Coord C2 exon
chr2:23253867-23254067
Length
1453 bp
Sequences
Splice sites
5' ss Seq
GAGGTGAAT
5' ss Score
5.37
3' ss Seq
ATACTCTTGTCCTGTCTCAGGAG
3' ss Score
7.62
Exon sequences
Seq C1 exon
GTTAAGCAGGGATTTGCTCGGCTGCAGGCGAAAGTACGCAGTCGGAAGCTCCATGAAGAGTACATGCGCAGACGGGCTGCCGCCATCGTTCTGCAAACACAGACCCGTGGCTTACTCGCCAGGAAAGAGCTGAAATCTAAAAAAGAGGCCGTGATTCTGCTGCAGGCACAGACCAGGGGACTGCTGGCCAGGAAAAGTCTCAAGAGAATGAAATCAGAG
Seq A exon
GTGAATTTAGAGTGGATCAGAACAACGTCTTTGTTGTTTTATATTTGATTCTAAAGTATGGAGGAATTATTTTGAAAGTCATTTCTCTTGCTCATCAAGCCTGCATTTATTTAAAGGCATAGTTCTCCCAAAAATGAAAATTCACATACTATTTACTCGCGTGTTTACAAACCTTTATGAGTTTCTCTGTTCTGTTGAACAGGAAGAGGATGCATTTTTCAAAATATATATTTTATTGTAGTTTTTACAGACATAGAAAAGCAAGGAACAGACAGACAAACCCATCATGAAACAAAAATCTTTGCAAAATGTTAAATAAAGCATAAAACAAGGAAAAATGGTGACATACAAGAGATACAGTATATGACCTAGAAAAAGACACAGTGATGTCACCTCACAGCAAGAAGGTCGCTGGTTCGACCCTCGGCTGGGTCAGTTGGCGTTTCTGTGTGGAGTTTGCATGTTCTCCCGTGTTCGTGTGGGTTTCCTTCGGATGCTCCGGTTTCCCCCACAGCCCAAACACATATGGTACAGGTGAATTAGGTAACCTAAATTGCCCATAGTGTATGTGTGTGAAAGAGAGAAGGGCATCCACTGCTTAAAAATGTGCTGGATAAGTTGGCGGTTCATTCCGCTGTGGCGACCCCAGACTAATAAAAGGACTAAGCCAAAAAGAAAATGAATGAATACTAGTCTTTATGGTGTATTAGAAAAAAAAATGAAATATTTTTAACCAACAAATCAGAATTTGAACACAGGACCAAAAAAGATGTGCTAAGGTCCCCTTAGCAGCTTTACACTTGTCGTATAAGGGGAAAACTGAGGGATAGAATTTACTAAGCTTCGTTTTAGAATAGGGAAGTCTGTGTAATATTTTGTTCTGAGTTAGATTGTGTCTAGAATTTATAGAACATAAATAAACAAATAAAAAGGAAGATACTTTGAAGAAAGCTAAAAACCTGTAACCCTTTACTTTCATGGTAGGAAAATGAATTGCCATAGAAGTCAATGGTCACAGGTTGCCAACATTCTTCAAAATATCTACTTTTCTGTTAAACAGAAGAAAGTCAAACTGGTTTGGAACAAGTAAAGGATGAAAGAATGTTCATTTCAATCTCTTTAAACAGAAATGCAGTGAAAGCTGTGTTATTATGCAATTATTATGCTATATTCTTGTAATATCAGCTCTGTATATTGATCCTTCAAAGAAAAAGAAGTATTTGCTAATCTATTTCTTTAGGATATTATTCTAAAGTCTAAAAAGAAAATGAATACATTTATTACAAGGGACACATTAAATTCATAAATTTTACTGAAAATTTTTATTGATGATTACACATGTAATAAATAATTACACATTTATTATTTTTAAATAAAAAGTCCTGAAAATAAAATCTATCGTGGTTTTCCACAAAATGAAGCGTTTATGACTGATACTCTTGTCCTGTCTCAG
Seq C2 exon
GAGTTTTTGACAGCACAAGAAAAACAAGCTCAGGAGCTGGCAGCTTTGGAGCTTCAGCAACGGTTGGAGGAGCTGCTCAGAAAAAACGAAGAAACTGCCAAATCCCAGAATAATGAAGATGAGGAAATGGTGGAGAATATTTTCAACTTCCTCCCGACTGCAATCGCAGGACAAGAGGGCCCGGCTCCTGAGGGTTTTGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000077201:ENSDART00000141621:19
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.434
Domain overlap (PFAM):
C1:
PF0061222=IQ=WD(100=28.8),PF0061222=IQ=WD(100=28.8)
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AAAGTACGCAGTCGGAAGCTC
R:
ATTGCAGTCGGGAGGAAGTTG
Band lengths:
353-1806
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]