DreINT0103412 @ danRer10
Intron Retention
Gene
ENSDARG00000032630 | neb
Description
nebulin [Source:ZFIN;Acc:ZDB-GENE-041111-216]
Coordinates
chr9:22998267-22998829:-
Coord C1 exon
chr9:22998643-22998829
Coord A exon
chr9:22998360-22998642
Coord C2 exon
chr9:22998267-22998359
Length
283 bp
Sequences
Splice sites
5' ss Seq
CTGGTCAGC
5' ss Score
3.85
3' ss Seq
TGTTTCGTTTTTTCTCAAAGACT
3' ss Score
6.65
Exon sequences
Seq C1 exon
GTCAAATACCACCAGGACTTTGAGAAGAGTAAGGGCAGCTTCACCCCCACCATCTCTGACCCAGTAACTGAGCGTGTGAAGAGGAACACCCAGGACTTCAGTGATATCAACTACCGTGGCATCCAGAGACGTGTGGTGGAGATGGAGAGGAAACGTGCCGAGGAGCACGATCAGGAGACCATCACTG
Seq A exon
GTCAGCTCACACAAATTATACACTCTAAACCAGGGCTTTCCAATCCTGCTCTTACAGTATGTCCAACAGACTTTAGCTTCAAAGAGCTCCAGCACTCTAGTCTCTCGTGAGTCTGGAGATGTATGTAATTAGGTTTAGAGCTAAAATCTGCAGGTTTGGACACCCCTGTTCTGTATGCACAAAAATGATGCCTTTACCTATAAGATGTGTTAAGAATCATACACAAATGATAATGAGTTATGAACGATAACATTCACTAAAACTGTTTCGTTTTTTCTCAAAG
Seq C2 exon
ACTTGCGTGTGTGGCGCACAAACCCTGGCTCTGTCTTTGACTATGACCCTGCCGAGGACAACATCCAGTCCAGAAGTCTTCACATGATGTCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000032630:ENSDART00000143888:131
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.683 A=NA C2=0.707
Domain overlap (PFAM):
C1:
PF0088013=Nebulin=PD(37.9=17.5)
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTCAAATACCACCAGGACTTTGAGA
R:
ACATCATGTGAAGACTTCTGGACTG
Band lengths:
277-560
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]