DreINT0103413 @ danRer10
Intron Retention
Gene
ENSDARG00000032630 | neb
Description
nebulin [Source:ZFIN;Acc:ZDB-GENE-041111-216]
Coordinates
chr9:22997933-22998359:-
Coord C1 exon
chr9:22998267-22998359
Coord A exon
chr9:22998065-22998266
Coord C2 exon
chr9:22997933-22998064
Length
202 bp
Sequences
Splice sites
5' ss Seq
CAGGTAGGC
5' ss Score
9.88
3' ss Seq
ATGTTTGTCCCGTTGTTCAGTTC
3' ss Score
7.04
Exon sequences
Seq C1 exon
ACTTGCGTGTGTGGCGCACAAACCCTGGCTCTGTCTTTGACTATGACCCTGCCGAGGACAACATCCAGTCCAGAAGTCTTCACATGATGTCAG
Seq A exon
GTAGGCTCACAACTCACACAACAGCTGTGTAAGGTCTTGGTAATCATCTGTCTGCACTGCTTGAGTTTAATTGGGTTACTGCAACATTCTGCATTCAGATATCTGTTTAACGTCTGCTAGATTGATACACTACAAACACTTCCTCAGCAGAAGGCATCTCTCAATAAGGAGATCATTTTAACATGTTTGTCCCGTTGTTCAG
Seq C2 exon
TTCAAGCCCAGCGCCGCAGCAAGGAGCACTCGCGCTCCACCAGTGCAATGAGTGGTGTGGGTGATGAGAAGTCAGAGGTGTCTGAGAACGTGGACCGCTACAGCAATGGCTTTGCCATCTCCGCTTTACACG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000032630:ENSDART00000143888:132
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.707 A=NA C2=0.697
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGTGGCGCACAAACCCTG
R:
CGTGTAAAGCGGAGATGGCAA
Band lengths:
216-418
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]