DreINT0106202 @ danRer10
Intron Retention
Gene
ENSDARG00000009341 | nrap
Description
nebulin-related anchoring protein [Source:ZFIN;Acc:ZDB-GENE-060616-224]
Coordinates
chr12:30478692-30479012:+
Coord C1 exon
chr12:30478692-30478799
Coord A exon
chr12:30478800-30478904
Coord C2 exon
chr12:30478905-30479012
Length
105 bp
Sequences
Splice sites
5' ss Seq
CAGGTGACA
5' ss Score
5.49
3' ss Seq
TATGCCTCTAATTCCTTTAGCAT
3' ss Score
6.89
Exon sequences
Seq C1 exon
GTAAAATATCAACAGAAATACGAGCAGGAGATTAAAGGCAAGGCCAGCACTGAAGCAGGAGCTGCTGAATTTGCTTTGGCCAAAGAAAACGCAGAGCACTTCAGCCAG
Seq A exon
GTGACACAGAGCTCTTCTGTATATTGTATATTGTGTGTGTTTTTATTTTTTTTATTTTTACATCATATACATTTTAATCATTATATATGCCTCTAATTCCTTTAG
Seq C2 exon
CATGCCTATACTGAAGAATATGAACAGCAGAGAGGCAAAGGGAGCTTTCCTGCTATGATTACCCCTGGATACCAGATGGCCAAGAAAGCACATGACATCGCCAGTGAT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000009341:ENSDART00000124920:10
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.194 A=NA C2=0.333
Domain overlap (PFAM):
C1:
PF0088013=Nebulin=PD(37.9=30.6),PF0088013=Nebulin=PU(53.6=41.7)
A:
NA
C2:
PF0088013=Nebulin=PD(39.3=30.6),PF0088013=Nebulin=PU(55.2=44.4)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TATCAACAGAAATACGAGCAGGAGA
R:
ATCACTGGCGATGTCATGTGC
Band lengths:
210-315
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]