DreINT0106228 @ danRer10
Intron Retention
Gene
ENSDARG00000009341 | nrap
Description
nebulin-related anchoring protein [Source:ZFIN;Acc:ZDB-GENE-060616-224]
Coordinates
chr12:30493883-30494291:+
Coord C1 exon
chr12:30493883-30493987
Coord A exon
chr12:30493988-30494183
Coord C2 exon
chr12:30494184-30494291
Length
196 bp
Sequences
Splice sites
5' ss Seq
GATGTAAGA
5' ss Score
5.46
3' ss Seq
CATTTTCTGTTGTATTTCAGGTT
3' ss Score
11.65
Exon sequences
Seq C1 exon
CGCTTATATAAAGAAAAGGGGGAGAACTTCAGGCACAACTACACCATTACTGCAGAGAGACCTGAAATCACCCAGGCCAAGATTAACGCTGCTAACTTCAGTGAT
Seq A exon
GTAAGATGAGCCCTCCAAGTCTATTTTTGAGTTTATGGATATTGCTGCATCATAAAATGCACTATTCTAAACTATTCACAATTTATAGTATATTTTAAAAATATTCTTAAGAAGAAATGCTTAAAATGCATTGCAAAAAGTGCACTTGTATCCAAGACGATAGTTGCAAGCTTTTACATTTTCTGTTGTATTTCAG
Seq C2 exon
GTTAAGTACAAGGAATCATGGAATGTGCTTCGAGCTCAGGGTTATAAACTCACCATGAAGGATATCCCCTTCCAAGCTGCCAAAGCATCCACCAGCATTGCTAGTGAT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000009341:ENSDART00000124920:34
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.200 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0088013=Nebulin=PD(37.9=31.4),PF0088013=Nebulin=PU(55.2=45.7)
A:
NA
C2:
PF0088013=Nebulin=PD(37.9=30.6),PF0088013=Nebulin=PU(55.2=44.4)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATAAAGAAAAGGGGGAGAACTTCAGG
R:
CTAGCAATGCTGGTGGATGCT
Band lengths:
202-398
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]