DreINT0106233 @ danRer10
Intron Retention
Gene
ENSDARG00000009341 | nrap
Description
nebulin-related anchoring protein [Source:ZFIN;Acc:ZDB-GENE-060616-224]
Coordinates
chr12:30496410-30496766:+
Coord C1 exon
chr12:30496410-30496514
Coord A exon
chr12:30496515-30496658
Coord C2 exon
chr12:30496659-30496766
Length
144 bp
Sequences
Splice sites
5' ss Seq
GATGTAAGC
5' ss Score
6.16
3' ss Seq
CTCTCTCTTTCACTTTGTAGAAA
3' ss Score
10.37
Exon sequences
Seq C1 exon
AGGATGTATAAGTCTGGGGACTCTGACTCCATGCACAAATACACATTGCATGCCGATCATCCTGACTTCATCAGAGCAAGGATCAACGCTCAGCAAATTAGCGAT
Seq A exon
GTAAGCTTGCTGTTATTCTGTGCTCTTACTTTTAGATGAATTACAGTAGCTCCATTTATGTTCCACATCGGTTGAATATTTTGATTGTCAGTGTACATCCTTTAATATTGTCCATGCTCTTTTTCTCTCTCTTTCACTTTGTAG
Seq C2 exon
AAAGTGTATAAAACATCAGGTGAGCAGGTGAAGTCTGCAGGTTATGACCTCAGGTTAGATGCCATTCCCTTCCAGACAGCCAAGGCCTCCAGAGAAATTGCAAGTGAT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000009341:ENSDART00000124920:39
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.143 A=NA C2=0.028
Domain overlap (PFAM):
C1:
PF0088013=Nebulin=PU(55.2=45.7)
A:
NA
C2:
PF0088013=Nebulin=PD(37.9=30.6),PF0088013=Nebulin=PU(55.2=44.4)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTCTGGGGACTCTGACTCCAT
R:
ATCACTTGCAATTTCTCTGGAGGC
Band lengths:
202-346
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]