GgaINT0041855 @ galGal4
Intron Retention
Gene
ENSGALG00000008914 | NRAP
Description
nebulin-related anchoring protein [Source:HGNC Symbol;Acc:HGNC:7988]
Coordinates
chr6:26993601-26995389:-
Coord C1 exon
chr6:26995285-26995389
Coord A exon
chr6:26993709-26995284
Coord C2 exon
chr6:26993601-26993708
Length
1576 bp
Sequences
Splice sites
5' ss Seq
GATGTAAGT
5' ss Score
9.11
3' ss Seq
TTCTTTTCTTTTCAACACAGAAA
3' ss Score
7.41
Exon sequences
Seq C1 exon
AGGCTGTACAAGGCTGGAGACTCTGAAGCCAGGCACAGGTATACCCTGCCTCCTGATCATCCAGATTTTATCCGAGCTCGACAGAATGCACTTAATATCAGTGAT
Seq A exon
GTAAGTAACAGAAGCTTTTACTTTGAGGAATTGACTTCGTAACAGTCATTAAAGAAAACAAATTTGCTTCAAAATGAAAAGCTTTCATGAATGTGTAGAAGTCAGGTTCTTTGAACCTGACATGAACTGAAAAAGGCAGAGCTTGTGGACTGCCAATACGCTGCTTGGCCGGGGAGGGAGGGATCAGGAAAGTTGTTTATTTTTCTCCTAAGAATTTGTTCTGCTTCCTAGGGCAAGAAGCTATTATTAAACATTTCAGTCCTAAAGTTTAGGAATAATTTCTTATAGATATAAATCTTTTTATTTACGTTTGATGAGGCAGAGAAAGAATCCCTCTCCTTCCTTGACTATATGATCTTTCGTCATTGAGTTGCAATGCTGTGTTGAACTGATCCCTTAGGTACAGTCCTCGACACGAGTGCAAATGAATCAGTGAGTGGAATTGGTTTAAAGAGATTTTCTTTCCACTTTACTTCATACAGTGGCACATGGTCTATCTCTAATAGTTGTGATCAAATTTGTCATCTTTAGTTGGAGGGTCAGAATGATCTTAGTTTGAGAAAAGTTTTGAGTTTCTCATTTGGTGGTAGGTCTTCTATATGTATAAGAAAGAAATAGGATTTAAGTTCTTTGATGTGACAGATGAAAAAAAAAAAGTAACATGGCAAAGGGGTGATTGCACACAAATGAAAGGTCAGCCCTAAACCTATTAACAAAGCATATGTTAATGTTTGCATTGGTTCTATCCAAGCTACTGATTCTGGCAGGACTTCTGGTATCTTAATTTACTTTCAAATTAAGCATCACCTGGCACTAAATCCATCAGTGAAACACACGAATATTTTTTTAAAAAATATGAAATAGATATTGTGCTGTTTTTCTCATATAATTGCCATCCTTCAAAAGGATGGATCAAAGTCTTCCAGAAAGCCAATCAATAAACACAGAAAGGGACAGCCAAAAATGTCTGCTAAGGTGCAACAGCTGGGCATCAGGACTGGAAGCTGTTGTAAGAGTGCTACTAAAGAAAGAAGAATCAGATTTAGAGAAATTTCTCTTCAAGATGTAAATGTATTTCACACCCACAAAGTCACATTAAATATGTTAGAACTGGCTGTTATGTCAGATGAGTTAATGAAGCCACATGTGGCTATGAAATAGAGACAGGAATCATTAGAAAAGCTTGAAATAACTAGTATTGAAATTATGATGCCATGAAACCCAAGACACTGCAAGTGGATGCATCAAAAGAGAGAATAAGAGTAATATCAATGCAATATTGATGTGACTGATGCATCACAGTTTCTAATAGAAATTAAATCCAATCAGAAACAACCACATTCATGTACAAAAGGAAACCCTAGTGGCATTTTTCAGATGAGACATTTTATCGTTATGGGGAATAGAAAACAAGTTGAAGGGCTGTAGAACTGGATCAAAACCTTGGAGATGGTACTGATGACTCTTGAAACAAACAAGCCCAGTAGGATAAAACATTTATTATTACAGTCATGGGACTACATTTAAGAAACTGCAAGGGGAAAATGACTGTTTTATTCTTTTCTTTTCAACACAG
Seq C2 exon
AAAGTGTATAAAACATCTTGGGAACAGACAAGAGCATCTGGCTATGATTTTAGGATTGACGCCATTCCATTCCAGACAGCAAAGGCTTCAAGAGAGATTGCTAGTGAT
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000008914:ENSGALT00000014498:38
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.314 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0088013=Nebulin=PD(21.7=14.3),PF0088013=Nebulin=PU(55.2=45.7)
A:
NA
C2:
PF0088013=Nebulin=PD(37.9=30.6),PF0088013=Nebulin=PU(55.2=44.4)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCTGTACAAGGCTGGAGACTC
R:
CACTAGCAATCTCTCTTGAAGCCT
Band lengths:
209-1785
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]