DreINT0111784 @ danRer10
Intron Retention
Gene
ENSDARG00000051915 | pde5ab
Description
phosphodiesterase 5A, cGMP-specific, b [Source:ZFIN;Acc:ZDB-GENE-060824-4]
Coordinates
chr7:57308099-57308407:+
Coord C1 exon
chr7:57308099-57308199
Coord A exon
chr7:57308200-57308329
Coord C2 exon
chr7:57308330-57308407
Length
130 bp
Sequences
Splice sites
5' ss Seq
GAAGTAGGT
5' ss Score
6.22
3' ss Seq
ATAATGCTGTCTGTCTGCAGAAA
3' ss Score
9.82
Exon sequences
Seq C1 exon
GGTAATCAGATTCTGAGTGGTCTTTCCCTGGATGAGTACAAGGCGACACTGAAGATGATTGAGAAGGCTATTTTGGCCACTGACCTGGCTGTCTACATGAA
Seq A exon
GTAGGTACCAGATGTCATGCACAGCCGGAAGCTCTTTGAGGGACCCTGTAAAGAGAGTTATTATTTGAGCACACTTGAGCTGATTATAATCCATTTTTTGTTGTCTGTTCATAATGCTGTCTGTCTGCAG
Seq C2 exon
AAAAAGAACAGAATTCTTCGAGCTAGCTAAGAACAGTCAGTTTGAATGGGAAGATGATTGCCACAGGGATCTATTAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000051915:ENSDART00000138188:16
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0023314=PDEase_I=FE(13.8=100)
A:
NA
C2:
PF0023314=PDEase_I=FE(10.9=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGTAATCAGATTCTGAGTGGTCT
R:
CTTAATAGATCCCTGTGGCAATCA
Band lengths:
179-309
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]