DreINT0111785 @ danRer10
Intron Retention
Gene
ENSDARG00000051915 | pde5ab
Description
phosphodiesterase 5A, cGMP-specific, b [Source:ZFIN;Acc:ZDB-GENE-060824-4]
Coordinates
chr7:57308330-57308571:+
Coord C1 exon
chr7:57308330-57308407
Coord A exon
chr7:57308408-57308507
Coord C2 exon
chr7:57308508-57308571
Length
100 bp
Sequences
Splice sites
5' ss Seq
AAGGTGAGC
5' ss Score
9.6
3' ss Seq
GGTGTTATGATTTTCTGTAGGTC
3' ss Score
7.36
Exon sequences
Seq C1 exon
AAAAAGAACAGAATTCTTCGAGCTAGCTAAGAACAGTCAGTTTGAATGGGAAGATGATTGCCACAGGGATCTATTAAG
Seq A exon
GTGAGCATTCAGTCAATAATGTAATGTGCTATTGTTTAGCTCACTGCATATATATATATATATATATATTTCCTAATTATGGTGTTATGATTTTCTGTAG
Seq C2 exon
GTCGATGCTGATGACAGCATGCGATATATCTGCTATTACAAAACCATGGCCAGTTCAGAAGAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000051915:ENSDART00000138188:17
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0023314=PDEase_I=FE(10.9=100)
A:
NA
C2:
PF0023314=PDEase_I=FE(8.8=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGAACAGAATTCTTCGAGCTAGCT
R:
CTTCTTCTGAACTGGCCATGGT
Band lengths:
138-238
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]